Electroacupuncture at Zusanli (ST36) Repairs Interstitial Cells of Cajal and Upregulates c-Kit Expression in Rats with SCI-Induced Neurogenic Bowel Dysfunction.
Electroacupuncture at Zusanli (ST36) Repairs Interstitial Cells of Cajal and Upregulates c-Kit Expression in Rats with SCI-Induced Neurogenic Bowel Dysfunction.
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电针足三里 (ST36) 修复 SCI 诱导的神经源性肠功能障碍大鼠 Cajal 间质细胞并上调 c-Kit 表达
DOI：
10.1155/2020/8896123
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Yang Y;Cheng J;Zhang Y;Guo J;Xie B;Zhang W;Zhu Z;Zhu Y
电针可改善脊髓损伤（SCI）所致神经源性肠功能障碍（NBD）大鼠结肠传输活动。Cajal间质细胞（interstitial cells of Cajal，ICCs）的功能和c-Kit的表达可能在这一过程中起重要作用。材料与方法。将36只SD道利大鼠随机分为假手术组、脊髓损伤组和脊髓损伤+电针组（双侧足三里穴，30 min/d，14 d）。观察ICCs超微结构的变化。分别采用免疫组化、Western blotting和RT-qPCR方法分析不同水平的c-Kit表达。 SCI后ICCs形态异常，c-Kit表达下调。电针大鼠在增加ICCs数量的同时，其超微结构也明显改善。他们还在蛋白质和基因水平上显示出c-Kit表达的更好改善。 脊髓损伤后结肠组织内ICC异常，c-Kit表达下调。电针足三里穴可通过修复结肠组织形态、增加ICC数量、上调c-Kit表达来改善结肠功能。
Electroacupuncture (EA) could improve colonic transit activity in rats with neurogenic bowel dysfunction (NBD) caused by spinal cord injury (SCI). The function of interstitial cells of Cajal (ICCs) and c-Kit expression may play essential roles in this process. Material and Methods. Thirty-six Sprague Dawley rats were randomized to the sham group, the SCI group, or the SCI + EA group (bilateral Zusanli, 30 min/day, 14 days). Changes in the ultrastructural morphology of ICCs were observed. The c-Kit expression on different levels was analyzed by immunohistochemistry, Western blotting, and RT-qPCR, respectively. Abnormal morphology of ICCs and downregulation of the c-Kit expression occurred after SCI. While the number of ICCs was increased, the ultrastructural morphology was improved significantly in EA rats. They also showed better improvement in c-Kit expression at both protein and gene levels. Abnormal ICCs in colon tissues and the downregulated expression of c-Kit could be observed after SCI. EA at Zusanli (ST36) could improve the colon function by repairing the morphology and increasing the number of ICCs and upregulating c-Kit expression.
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影响因子：
29.4
作者：
He, CL;Burgart, L;Farrugia, G
通讯作者：
Farrugia, G
影响因子：
--
作者：
Cheng J;Wang X;Guo J;Yang Y;Zhang W;Xie B;Zhu Z;Lu Y;Zhu Y
通讯作者：
Zhu Y
电针治疗通过激活迷走神经部分促进术后肠梗阻的恢复时间，但不能调节局部炎症
影响因子：
4.6
作者：
Fang, Jun-fan;Fang, Jian-qiao;Liu, Zhe
通讯作者：
Liu, Zhe
电针 ST37 和 ST25 通过影响兴奋性和抑制性神经元，通过肠神经系统对结肠运动产生不同的影响
影响因子：
3.5
作者：
Liang, C.;Wang, K. -Y.;Xu, B.
通讯作者：
Xu, B.
DOI：
10.1155/2013/543174
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Liu Z;Wang W;Wu J;Zhou K;Liu B
通讯作者：
Liu B