在体外受精治疗中常观察到不良精子质量可以导致植入前胚胎发育阻滞，但其作用机制一直不甚清楚。目前已明确正常的胚胎基因组活化（EGA）对于囊胚的发育至关重要，但在活化过程中精子基因组起何作用，而不良精子质量是否会通过影响正常的EGA而导致胚胎发育的阻滞是亟待解答的问题。本项目拟通过对常规体外受精和孤雄激活的不同发育阶段胚胎进行单细胞RNA测序和全外显子测序，结合胚胎的转录本信息与亲缘基因组单核苷酸多态信息进行生物信息学分析来确定不同EGA阶段亲缘基因组的贡献，进而明确精子基因组在EGA启动过程中发挥的作用。并通过对不同类型异常精子受精后胚胎EGA的启动及胚胎发育潜能的分析，研究精子质量、EGA和胚胎发育潜能三者之间的关系。本研究对于深入理解精子因素对胚胎发育潜能的影响特别是对EGA启动的调控作用具有重要意义。

It often observed that poor sperm quality can lead to preimplantation embryo development arrest during in vitro fertilization treatment, but the mechanism of it has been unclear. It has been clear recently that normal embryonic genome activation (EGA) is essential for the development of the blastocyst. However whether the sperm genome activation process played any role, or poor sperm quality by affecting normal EGA to lead embryonic development arrest is an urgent question. This project intend to use single-cell RNA sequencing and exon sequencing to test embryos from different developmental stages by conventional IVF and activation of haploid androgenesis, combining with the information of embryonic genetic transcription and the information of genome single nucleotide polymorphism to take bioinformatic analysis to determine the contribution of different stages of genetic genome in EGA, and then to know the role of genome in sperm EGA activation. And through the analysis of EGA which fertilized with different types of abnormal sperm and the potential of embryonic development to research the relationship of sperm quality, EGA and the potential of embryonic development. This study is particularly important, especially for the regulation of EGA, to deeply understand the factors of sperm affecting on the potential of embryonic development.