目前，国内农产品存在规模小、分散、晾晒方法不科学等问题，且商品化的酶联免疫试剂盒、薄层色谱、胶体金免疫层析等真菌毒素快检方法的假阳性率普遍较高，影响了食品生产企业的自检和监管机构的监测效果。本项目将设计、制备闭合式BPE，从而将反应体系与信号测量体系进行物理隔离；在闭合式BPE的阴极端沉积金纳米粒子，使其降低电极反应的电势，增大电极反应的电流；采用共价作用或非共价作用实现真菌毒素的抗体或核酸适体在金纳米粒子上的组装固定，利用抗体或核酸适体和真菌毒素之间的特异性识别，构建农产品中真菌毒素的检测方法；开发闭合式BPE可视化阵列芯片，实现真菌毒素快速、灵敏的成像检测，弥补国标方法检测真菌毒素耗时、成本高、工作量大等不足，降低劳动强度，提高检测效率，适应现代批量筛查的需求。

Mycotoxins are usually not destroyed by food grain processing or food cooking by the heating. At present, the domestic agricultural products are small-scale, scattered, unscientific drying methods. Additionally, it is generally high for false-positive rates of commercial ELISA, TLC and colloidal gold immunochromatography, affecting the self-inspection results of food production enterprises and monitoring results of regulatory agencies. Therefore, it is of vital importance to develop efficient advanced methods for rapid detection of mycotoxins and to achieve miniaturized, integrated and multichannel detection of instrument. It is easier to build the detection device for bipolar electrode (BPE) and the external power supply without wires connected. Additionally, BPE physically separates the reaction system from the signal measurement system. The analytes do not participate in the ECL reaction of the anode, avoiding the direct contact between the photoactive molecules and the complex reaction system, greatly expanding applied range of the ECL. At the same time, BPE is also easily arrayed, capturing the ECL signal on each sensing interface with a CCD for high-throughput detection increases the amount of information obtained in a single analysis. The perfect combination of BPE and ECL technology has many of these advantages, ideal for analysis of mycotoxins in more complex agricultural products.