低聚木糖干预沙门氏菌粘附素和侵袭素表达抑制鸡肠黏膜损伤的机制

鸡副伤寒是鼠伤寒沙门氏菌引起、以肠炎和败血性为病症的传染病。项目组前期研究发现，低聚木糖调控与沙门氏菌粘附和定植肠黏膜相关基因表达，实现其对肠黏膜损伤的抑制。项目分离粘附素和侵袭素，筛选关键因子，构建鼠伤寒沙门氏菌关键粘附素和侵袭素减毒变异株，粘附素、侵袭素及减毒株攻毒肠上皮细胞和SPF鸡，通过肠黏膜损伤、代谢组和免疫组测定明确沙门氏菌关键粘附素和侵袭素及其致病机制。低聚木糖处理沙门氏菌，粘附素和侵袭素测定，代谢组和转录组研究低聚木糖干预粘附素和侵袭素表达及其机制；低聚木糖处理的沙门氏菌及其粘附素与侵袭素感染肠上皮细胞和SPF鸡，细胞和肠黏膜损伤效应测定，代谢组和免疫组测定细胞和肠黏膜损伤相关代谢因子和免疫因子差异表达，分析其损伤干预机制，解析低聚木糖干预沙门氏菌粘附素和侵袭素表达抑制鸡肠黏膜损伤的机制。研究结果对明确低聚木糖抑制沙门氏菌危害作用、解析其机制具有重要的学术价值和应用前景。

Chicken paratyphoid, a serious threat to bird septic infection is caused by Typhimurium. Our preliminary study showed that xylo-oligosaccharides regulate the expression of genes associated with adhesion and colonization of Salmonella, and effectively inhibit the damage of intestinal epithelial cells from bacterial. This project intends to isolate adhesin and invasive factors and screen key factors to construct Salmonella key adhesins and attenuated variants of Salmonella attacking enterocytes and SPF chickens. The key adhesion proteins and invasive elements of Salmonella and their pathogenic mechanisms were identified through the measurement of intestinal mucosal injury, metabolomics and immunological analysis. Xylo-oligosaccharide interferes with Salmonella proliferation, metabolism and the expression of virulence factors by metabolome and transcriptome studying on the expression and mechanism of xylo-oligosaccharides interfering with adhesion and invasive factors. Adhesins, invasive factors and their attenuated variants infect on chicken epithelial cells and SPF chickens. By testing the effects of cell and intestinal mucosal damage, and using metabolome and immunological assays to determine the differential expression of metabolic factors and immune factors associated with cell and intestinal mucosal damage, the host metabolism and immune mechanisms of chicken intestinal epithelial cells and mucosal lesions will be analyzed. The mechanism on xylo-oligosaccharides was based on the intervention of Salmonella adhesion and invasion to protect intestinal mucosa from injuring in chickens. This research has important academic value and practical significance for analysis of the mechanism of xylo-oligosaccharides in inhibiting the risk of Salmonella.