高分子凝胶材料作为重要的生物医用材料近年来得到了科学界的高度关注，其在组织工程、药物传递、细胞培养基质及生物标志物富集检测等方面的研究取得了令人瞩目的进展。与目前的生物医用需求相比，高分子纳米水凝胶微球的制备技术还远不能满足实用要求，且发展缓慢。本申请针对当前高分子纳米水凝胶制备存在的问题，提出了一种制备高分子纳米水凝胶的新技术- - 回流沉淀聚合技术，实现对单分散高分子纳米水凝胶微球的高效制备及结构控制，并从原理上阐明其形成机制及关键控制参数；在此基础上，进行具有特定功能结构（如空心及核壳等）的有机无机磁性复合凝胶微球的制备研究，获得具有生物相容和可生物代谢的凝胶微球，可满足靶向药物载体、多功能造影剂及翻译后修饰蛋白（生物标志物）富集分离的功能要求，并在生物医用方面进行一些探索研究。

Polymeric hydrogel, a kind of significant biomaterials, has attracted much attention in scientific community. A great progress of the application of polymeric hydrogels has been archived in the biomedical fields, such as scaffolds for tissue engineering, vehicles for drug delivery, matrix for cell culture technique, enrichment and detection for biomarkers. Compared with the demands of the biomedical applications, the preparation techniques of polymeric nanohydrogels can't meet the requirements of the application, and the development is quite slow. Under this condition, we develop a new preparation method - reflux precipitation polymerization - for high efficient preparation of polymeric nanohydrogels, which will realize the facile preparation and structured control of the monodispersed polymeric nanohydrogels, the formation mechanism and the key preparation parameters also will be investigated. Based on the above studies, the well-defined magnetic composite microspheres with biodegradability and biocompatibility will be fabricated, these composite nanohydrogels will fulfill the demands for targeted drug delivery system,contust agents or enrichment of functional proteins (biomarkers), the relative investigation for drug delivery and biomarker detection also will be conducted in this project.