Melon short internode (CmSi) encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling.
Melon short internode (CmSi) encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling.
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甜瓜短节间 (CmSi) 编码 ERECTA 样受体激酶，通过生长素信号调节茎伸长
DOI：
10.1038/s41438-020-00426-6
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发表时间：
2020-12-01
影响因子：
8.7
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Yang S;Zhang K;Zhu H;Zhang X;Yan W;Xu N;Liu D;Hu J;Wu Y;Weng Y;Yang L
株高是直接决定植物结构的最重要的农艺性状之一，紧凑或矮化植物可以允许增加种植密度和土地利用率以及增加抗倒伏性和经济产量。在不同的甜瓜品种中已经鉴定出至少4个矮秆/半矮秆基因，但没有一个被克隆出来，并且对甜瓜节间伸长的分子机制知之甚少。在这里，我们报告的第一个半矮秆基因短节间（CMSI），它编码ERECTA样受体激酶调节节间伸长甜瓜的图位克隆和功能特性。时空表达分析表明，CmSI在节间伸长过程中在主茎的维管束中表现出高表达。CmSI的表达水平与不同甜瓜品种的茎长呈正相关。CmSI在拟南芥和黄瓜中的异位表达表明CmSI在这两个物种中都是节间伸长的正调节因子。植物激素定量和转录组分析表明，生长素含量和参与生长素信号通路的一些基因的表达水平在半矮秆突变体中发生了改变，包括几个众所周知的生长素转运蛋白，如ABCB家族成员和PIN-FORMED基因。甜瓜生长素极性转运蛋白CmPIN 2通过蛋白质-蛋白质相互作用分析被鉴定为与CmSI物理相互作用以调节生长素信号传导。因此，CmSI功能在生长素依赖的调节途径，以控制甜瓜节间伸长。我们的研究结果表明，ERECTA家族基因CmSI通过生长素信号调节甜瓜茎的伸长，这可以直接影响生长素的极性运输。
Plant height is one of the most important agronomic traits that directly determines plant architecture, and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic yield. At least four dwarf/semidwarf genes have been identified in different melon varieties, but none of them have been cloned, and little is known about the molecular mechanisms underlying internode elongation in melon. Here, we report map-based cloning and functional characterization of the first semidwarf gene short internode (Cmsi) in melon, which encodes an ERECTA-like receptor kinase regulating internode elongation. Spatial-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode elongation. The expression level of CmSI was positively correlated with stem length in the different melon varieties examined. Ectopic expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both species. Phytohormone quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant, including several well-known auxin transporters, such as members of the ABCB family and PIN-FORMED genes. A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin signaling. Thus, CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in melon. Our findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling, which can directly affect polar auxin transport.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
10.5
作者：
Lin G;Zhang L;Han Z;Yang X;Liu W;Li E;Chang J;Qi Y;Shpak ED;Chai J
通讯作者：
Chai J
OsPIN2 的过度表达通过抑制 OsLAZY1 导致分蘖数量增加、角度增加和株高缩短
影响因子：
13.8
作者：
Chen, Yingnan;Fan, Xiaorong;Xu, Guohua
通讯作者：
Xu, Guohua
影响因子：
3.1
作者：
Hwang, Jihyun;Oh, Juyoul;Park, Younghoon
通讯作者：
Park, Younghoon
DOI：
10.1111/pce.12377
发表时间：
2014-10
期刊：
Plant, cell & environment
影响因子：
--
作者：
Ayano M;Kani T;Kojima M;Sakakibara H;Kitaoka T;Kuroha T;Angeles-Shim RB;Kitano H;Nagai K;Ashikari M
通讯作者：
Ashikari M