油脂脱臭馏出物中PAHs、OPAHs和ClPAHs的酶解机制及安全评估

多环芳烃（PAHs）属国际公认的高毒持久性有机污染物，其氧化和单氯代衍生物（OPAHs和ClPAHs）较母体PAHs危害更大。因PAHs的高度亲脂性，环境中的PAHs极易向油料、油脂中迁移聚集，并在油脂精炼后的脱臭馏出物中高度富集。项目组基于对餐厨废油中PAHs成功酶解的预实验基础，拟针对脱臭馏出物PAHs高污染现状，以生育酚不变、PAHs及其衍生物降解去毒为目的，研究脱臭馏出物中PAHs、OPAHs和ClPAHs的酶解效能和机制。通过同位素标记稀释、GC-QQQ-MS、LC-ESI-TOF-MS/MS、制备HPLC以及UPCC/MS等测定酶解产物，绘制PAHs、OPAHs和ClPAHs的酶解路径图；综合采用 QSAR/QSPR、Microtox及细胞毒性评估等技术，评估PAHs降解产物的安全性。研究结果将为油脂工业乃至其他领域中PAHs及其衍生物的绿色控制、降污节能等提供科学依据。

Polycyclic aromatic hydrocarbons (PAHs) are internationally recognized as highly toxic persistent organic compounds. Oxygenated and single chlorinated PAHs (OPAHs and ClPAHs) are more harmful than their parent PAHs. Due to high lipophilic nature, PAHs can easily migrate and aggregate in oil seeds and edible oils, and heavily enrich in oil deodorizer distillates during oil refining. Based on our successful pre-experiments on enzymatic degradation for PAHs in waste cooking oils, this study aims at both PAHs detoxification and tocopherols remaining. The enzymatic degradation efficiency and mechanism for PAHs in oil deodorizer distillates will be disclosed. Multi-analytical methods, including isotope dilution analysis (IDA), GC-QQQ-MS, LC-ESI-TOF-MS/MS, preparative HPLC, and UPCC/MS, will be utilized to determine degradation products. Enzymatic decomposition pathway for PAHs, OPAHs and ClPAHs will be proposed. Multiple methods, including QSAR/QSPR risk assessment, Microtox test and cytotoxicity, will be carried out to evaluate the toxicity of degradation molecules. The results in this study may provide both scientific basis for edible oil industry and other area on PAHs green control, pollution-reduction and energy-saving.