小麦条锈病是小麦生产中最重要的流行性病害,严重威胁着我国乃至全世界的小麦安全生产。现阶段生产中存在的主要问题是抗条锈病品种缺乏持久抗病性。研究发现高温抗条锈性是一种低反应型的持久抗病性，由主效基因控制，且易于鉴选和利用。我国具有丰富的高温抗条锈性小麦品种资源，这些品种具有重要的利用价值，但关于高温抗条锈性的分子机制研究迄今仍为空白。本项目拟在前期建立的小麦温度转换培养法及高温抗条锈性的表达规律和生化机制的基础上，以小偃6号和兰天1号小麦品种与条锈菌CYR32为对象，采用RNA-seq技术，分析小麦在高温处理前后的转录组变化，结合生物信息学分析、qRT-PCR分析及病毒诱导的基因沉默技术进行基因功能验证，了解高温抗病性表达过程中的防卫反应激活、信号传递和基因调控规律，揭示小麦品种高温抗条锈性的分子机制，以期为最终实现高温持久抗条锈性基因的克隆和利用奠定基础。

Wheat stripe rust, which treatens wheat production all over the world, is one of important epidemic diseases. Currently, main problem in wheat production is no durable resistance cultivar among resistant cultivars. High temperature resistance of plant is a kind of durable resistance of low infection type, which is controlled by major gene, easy to be screened, identified, and used.The cultivars with high temperature resistance are very rich in China. It is still unclear on the molecular mechanism of high temperature resistance of wheat cultivars to wheat stripe rust. In this research, the transcriptome of Xiaoyan 6 and Lantian 1 treated under high and normal temperature will be sequenced and analyzed using RNA-seq and bioinformatic technologies based on our previous results of temperature transformation method, expression characteristics, and biological and chemical mechanisms of high temperature resistance to stripe rust. The key genes related to high temperature resistance to stripe rust will be cloned. The functioins of these genes will be verified by virus-induced gene-silencing system. The defense responses, signaling, and gene regulation during high temperture resistance expressed to wheat stripe rust will be understood. The molecular mechanism of high temperature resistance to stripe rust will be revealed. This research will make a base for gene cloning and usage of high temperature durable resistance to wheat stripe rust.