条锈病是小麦上最重要的流行性病害之一，严重威胁着我国小麦安全生产。在我国小麦条锈病防控中，现阶段存在的主要问题是抗病品种缺乏抗病持久性。小麦品种的高温抗条锈性是指在相对较高环境温度下表达的一种低反应型抗病性，是一种非小种专化的持久抗病性，由主效基因控制，易于鉴选和利用。本项目组以典型的高温抗条锈性品种小偃6号与条锈菌CYR32为模式体系，开展了其高温抗条锈性转录组分析，推测受体类激酶可能是参与小偃6号在条锈菌和高温诱导下从亲和性（感病）向非亲和性（抗病）转变的关键酶，其中TaCRK10在条锈菌和高温诱导后表达量上调倍数最高，沉默该基因植株的高温抗病性显著下降。本研究拟在此基础上，深入研究TaCRK10的功能及所处的信号通路，明确在条锈菌和高温诱导下TaCRK10的调控网络，分析其参与调控小偃6号实现从亲和性向非亲和性转化的分子机制，为进一步揭示小偃6号高温抗条锈病的分子机理奠定基础。

Wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), one of the most important epidemic diseases in wheat (Triticum aestivum) worldwide, seriously threatens the safety of wheat production in China. Currently, cultivars absent durable resistance to stripe rust is the main problem in stripe rust control. Wheat high-temperature resistance to Pst is a kind of low response type, race-non-specific and durable resistance, which is induced by relative high temperature and controlled by major genes, and easy to be identified and utilized. In our Lab., Xiaoyan 6 (XY 6), which is a typical high-temperature resistance cultivar, and Pst CYR32 were chosen as model materials to analysis the transcriptome. We speculated that the receptor like kinases may be the key enzymes involved in transformation from compatibility (susceptible) to incompatibility (resistance) induced by Pst inoculation and high-temperature in XY 6. Among them, the up-regulation level of TaCRK10 was the greatest, and silencing TaCRK10 led to significantly reduced high-temperature resistance of XY 6 to Pst. Based on these results, we will study the function and signal pathway of TaCRK10, clarify the network regulated by TaCRK10 under Pst and high-temperature treatment, analyze the molecular mechanism of transformation from compatibility (susceptible) to incompatibility (resistance) in XY 6 in order to establish foundation for revealing the molecular mechanism of high-temperature resistance of XY 6 to Pst in the future.