生物组织对光的衰减作用是光在组织内部穿透深度受限的主要原因。在对肿瘤的光热治疗（PTT）中，也会因光的损耗导致治疗效果降低。采用近红外二区（NIR-Ⅱ）光源有望突破当前NIR-Ⅰ区光源的组织传播限制，提升对深层病灶组织的PTT治疗效率。本项目拟建立对NIR-Ⅱ区响应的氧化钼基纳米体系，应用于肿瘤的NIR-Ⅱ诊疗。同时，半导体材料也是近年在表面增强拉曼散射（SERS）研究领域的新星。氧化钼纳米结构同样也展示出很好的SERS活性。探索所构建氧化钼基纳米结构的Bio-SERS应用潜力，并实现在SERS引导下的肿瘤NIR-Ⅱ光热治疗。对比分析NIR-Ⅰ和NIR-Ⅱ激光对深层病灶组织的光热杀伤，以判定NIR-Ⅱ PTT的效果。最后，利用Bio-SERS技术结合其他生化手段研究氧化钼基纳米结构与细胞的相互作用机制和NIR-Ⅱ PTT分子机制。

The attenuation of light in biological tissue is the main reason for the limited penetration of light in the tissue, also reduces the efficacy of photothermal therapy (PTT). The use of light source in the second near-infrared (NIR-Ⅱ) window is expected to break through the limitation of tissue transmission of the current NIR-I light source, and improve the PTT efficiency for the deep pathological tissue. This project aims to establish a NIR-Ⅱ responsive and molybdenum oxide (MoOx)-based nano-system for NIR-Ⅱ tumor theranostics. Meanwhile, as a new star in the field of surface-enhanced Raman scattering (SERS) in recent years, MoOx nanostructure exhibits good SERS activity. This study will explore the potential of the Bio-SERS application of the MoOx-based nanostructures and to achieve the NIR-Ⅱ photothermal therapy under the guidance of SERS technique. In order to determine the effect of NIR-Ⅱ PTT, the photothermal damage of NIR-I and NIR-Ⅱ laser on the deep pathological tissue will be compared. Finally, Bio-SERS technology and biochemical methods will be adopted to study the mechanism of interaction between MoOx nanostructures and cells, as well as the molecular mechanism of NIR-Ⅱ PTT.