南方根结线虫是重要的专性内寄生植物病原线虫，可寄生二千多种植物，据估计，全世界因其危害造成的损失高达数百亿美元，被称作最具破坏性的植物病原物，目前仍缺乏安全有效防治措施，揭示线虫寄生致病基因的功能是防治线虫的重要基础。本研究拟利用原位杂交、发育表达模式分析、免疫组织定位和植物细胞瞬时表达、酵母双杂交、Gst pull down和BiFC、植物转基因和基因芯片及RNAi等技术明确该线虫新基因MiMsp40产物的表达部位及在不同虫龄的表达量差异，获得MiMsp40与拟南芥的互作蛋白，分析转MiMsp40基因的拟南芥其形态、线虫敏感性和基因表达变化，利用RNAi和拟南芥缺失突变体研究其功能，明确该新基因是否具有调控植物代谢或抑制其免疫反应、促进线虫与植物建立寄生关系的功能；研究结果将为进一步阐明和完善根结线虫致病分子机理提供重要数据，为寻找防治线虫新靶标及制定防治新策略提供依据。

South root knot nematode (Meloidogyne incognita) is an obligate, sedentary, endoparasitic plant nematode. Its host range was more than two thousand plants. It was estimated annual lose causing by root knot nematodes will cost over 50 billion US dollar in the world. This plant parasitic nematode is called as one of the most destructive plant pathogen. Unfortunately, the environmental-friendly approach for management of this nematode is still lacking. Understanding of the function of parasitic genes will help to establish an important foundation for control of this nematode. In this research, we will using a series of techniques and methods such as in situ hybridization and immunohistochemistry technique, subcellar location in onion epidermis, real time PCR, yeast two hybridization, and Gst pull down and BiFC, plant transgenic technique, DNA microarray, in vitro and in vivo RNAi and rescue of mutants of interacting proteins of Arabidopsis thaliana to explore the function of pioneer parasitic gene designed MiMsp40 isolated from Meloidogyne incognita, we expect to obtain the gene expression organ, expressing differential of developmental stages of nematode, Screening and validation of the protein of Arabidopsis thaliana interacting with MiMsp40, investigation of the morphological changes and sensitivity of the plant of Arabidopsis thaliana over-expressed MiMsp40 to nematode infection, and gene expression differential of the transgenic Arabidopsis thaliana, and survey of plant pathogenesis after RNAi MiMsp40. We expect all our results will ascertain if the MiMsp40 of south root knot nematode has a role to regulate host plant metabolism or suppress immune reaction of plant,and then helping root knot nematode to establish parasitic relationship with its plant host. Our results will provide some basic information for further elucidating the molecular mechanism of parasitism and pathogenesis of root knot nematode. And the results will also pave some theoretical basis for screening new RANi target gene of nematode and working out a new strategy for control of root knot nematodes.