象耳豆根结线虫是源自我国海南、致病力强、危害严重的病原物，现正在我国不同区域扩散蔓延，严重影响疫区蔬菜瓜果等作物的安全生产，目前缺乏安全有效防治措施，解析线虫效应子功能是获得防治新靶标及制订防治新策略的前提。本研究拟利用发育表达模式分析、原位杂交、Western blot、免疫组织定位和植物细胞瞬时表达、酵母双杂交、Co-IP和BiFC、植物转基因、转录组和质谱分析及RNAi技术等生物化学、分子生物学和植物病理学手段明确MeSP1新效应子在不同虫态表达差异和组织表达部位，获得MeSP1与植物的互作蛋白，探明转MeSP1基因拟南芥其形态、基因表达通路及对线虫敏感性的差异，利用RNAi和拟南芥缺失突变体研究其功能，探究MeSP1翻译后修饰如何影响与植物的互作及效应子功能。研究结果将为进一步阐明根结线虫调控植物免疫反应的机理提供重要数据，为寻找防治新靶标并制订防治新策略提供理论依据。

Root knot nematode Meloidogyne enterolobii original isolated from Hainan province. It is a high virulence and the most devastating plant-parasitic nematode, causing vast economic loss in China. It is spreading rapidly from its original Hainan province to north China recent. This nematode is becoming a key factor to limit safe production of vegetable and melons fruits in infestation area. However, there are no safe and effective control approaches for management of this root knot nematode so far. Functional analysis of nematode effectors will contribute to develop new targets and strategies for its control. The study here will use the methods and techniques including in situ hybridization, developmental expression pattern analysis, Western blot, immunohistochemistry localization, transient expression via virus, yeast two-hybrid system, Co-IP, BiFC, plant genetic transformation, transcriptional analysis, RNAi, Arabidopsis thaliana mutant, etc., and explore the localization of effector, gene expression at different stages of nematode, interaction with the proteins in A. thaliana, and the morphology, gene expression differences and susceptibility to nematodes by overexpression of MeSP1 in A. thaliana. And we also will investigate how to manipulating plant immunity such as PTI and ETI. These results will ascertain the function of MeSP1 in the mechanism of regulating plant immunity of plant after invasion into plant and how to help to establish the parasitism of the nematode, and exploring the relationship between post-translational modification of MesSP1 and its function. In summary, the results will support to find a new target gene for nematode control and then development of the new nematode control strategy.