qLGY3控制水稻粒长和提高产量的分子机制研究

增加千粒重是实现水稻高产育种的有效途径之一。然而，水稻籽粒大小（粒长和粒宽）是受多基因和环境影响的复杂数量性状，其分子调控机制目前仍不很清楚。利用细长粒型的美国水稻品种L204构建的CSSL群体，QTL分析检测到了一个与细长粒型和产量增加相关的主效QTL：qLGY3（Long grain and high yield）。通过图位克隆获得了候选基因LGY3，发现LGY3蛋白能与水稻高产dep1基因编码蛋白互作。在此基础上，本项目拟利用RNA-seq分析LGY3可能调控的下游基因；分析LGY3与其它粒型基因（如GS3和GW8等）之间的遗传互作关系；鉴定LGY3互作蛋白并研究其生物学功能；重点解析dep1和LGY3蛋白互作协同提高穗粒数和千粒重的分子机制，为水稻高产分子设计育种奠定理论基础。

Rice yield traits consist of three key components, including tiller number per plant, grain number per panicle and grain weight, which are controlled by quantitative trait loci. Recently, we identified a major QTL,qLGY3, which was related to long slender grain and high yield on the chromosome 3 in rice. In this proposal, we will isolate the LGY3 gene through map-based cloning and analyze the molecular characterization of LGY3 and its regulatory networks using RNA-seq technology. Furthermore, we will identify the LGY3-interacting proteins via yeast-two hybrid screening. More importally, the molecular function of the interaction between DEP1 and LGY3 protein will be analyzed in-depth.