食品安全问题是关系国计民生的重大公共卫生问题，本项目拟采用碳点及复合物等过氧化物模拟酶代替天然生物酶，以食品中的致病微生物及所产生的毒素作为研究对象，开展金黄色葡萄球菌及肠毒素的现场、快速检测方法研究。拟制备高催化活性的过氧化物模拟酶，将模拟酶自组装成膜作为固定生物分子的介质，用多种表征方法研究模拟酶膜的组成及形貌对其催化活性的影响规律，实现模拟酶的可控自组装。在模拟酶膜表面固定抗体等生物分子，通过免疫反应特异性识别目标检测物来调节模拟酶膜的催化能力，从而调控模拟酶催化分解双氧水和双氧水与氯金酸反应生成有色金溶胶这对竞争反应，放大检测信号，实现金黄色葡萄球菌及肠毒素的高灵敏度检测。探求这种检测方法的信号增强机制及规律，以食品及原料等实际样品研究该方法的选择性和灵敏度，并对各项性能进行全面评估。这种检测方法为预防食源性疾病的暴发、流行及追踪污染源提供科学依据，具有重要的科学意义。

Food safety is a major issue related to people's health and the national economy and the people's livelihood. The project is going to employ enzyme mimetic instead of natural biological enzyme to determine food borne pathogenic microorganism and toxin in the food. A rapid assay for detection of Staphylococcus aureus and its enterotoxin will be designed. Firstly, high catalytic activity of enzyme mimic such as carbon dots and carbon dots composites will be synthesized. Then, these nanoparticles will be self assembled into a layer of efficiently catalytic membrane. A variety of characterization will be performed to investigate enzyme mimic membrane composition and morphology, which faciliate to realize controllable self-assembly of enzyme mimic. Secondly, biological molecules such as antibodies will be modified on the enzyme mimic membrane surface. The immunoreaction will be performed to realize selective recognition of analytes. A coulp of competition between enzyme mimic catalytic decomposition of hydrogen peroxide and hydrogen peroxide and HAuCl4 will be introduced to display different colors, which establish a highly sensitive and colorimetric assay to detect Staphylococcus aureus and its enterotoxin. Thirdly, the practical food samples will be emplolyed to study the selectivity, sensitivity of the resulted assay. This rapid detection assay provides scientific basis for the prevention of foodborne disease outbreak, epidemic and tracking pollution sources, and has important public health and scientific significance.