circ0008157海绵吸附miR-197-5p和miR-335-3p抑制黑色素瘤血管生成的研究

前期circRNA芯片发现circ0008157在黑色素瘤细胞中低表达，预实验发现circ0008157通过负向调控VEGF-A抑制黑色素瘤发生发展，而VEGF-A是促血管生成的关键因子。生物信息学分析及预实验microRNA芯片结果提示miR-197-5p及miR-335-3p与circ0008157均存在结合位点，且共同靶向CYP4Z1。本课题将以circ0008157调控miR-197-5p、miR-335-3p抑制黑色素瘤血管生成为主轴，从分子、细胞和动物实验三个层次探讨circ0008157海绵吸附miR-197-5p、miR-335-3p导致CYP4Z1蓄积抑制血管生成的机制；最后回归临床，探讨黑色素瘤中circ0008157、miR-197-5p、miR-335-3p及血管生成相关分子的表达量的临床意义。

In our previous circRNA chip experiment on screening circular RNAs, we found circ0008157 was reduced expression in melanoma cells. Preliminary study showed circ0008157 inhibited progress of melanoma cells via silencing VEGF-A,the most important factor in angiogenesis. According to bioinformatics software, circ0008157 is predicted to be a target of miR-197-5p and miR-335-3p. And CYP4Z1 is the target gene of these two microRNAs. In this study, we aim at proving the angiogenesis phenotype suppressed by circ0008157 through sponging miR-197-5p and miR-335-3p in melanoma. We will explore the relationgship among circ0008157, miR-197-5p, miR-335-3p, CYP4Z1 and angiogenesis level of melanoma cells in vitro. Finally, we will discuss the value of circ0008157、miR-197-5p and miR-335-3p and angiogenesis related factors in melanoma patients by analyzing the collected clinical samples.