NF-Y型转录因子通过在转录水平上对下游逆境响应基因进行调控，在介导植株抵御干旱胁迫逆境中发挥着重要作用。本课题在前期工作基础上，以在小麦中鉴定的10个NF-YB转录因子家族基因（TaNF-YB1~TaNF-YB10）为基础，采用实时定量PCR技术系统研究供试基因应答干旱逆境的时空表达模式；通过检测供试基因与绿色荧光蛋白基因（GFP）融合蛋白分布部位，明确供试基因编码蛋白亚细胞定位特征；利用酵母双杂交辅以BiFC技术，鉴定供试基因编码NF-YB蛋白可能互作的NF-YC和NF-YA组分，阐明小麦NF-Y转录因子亚基构成模式；通过基因过表达和表达干涉技术，鉴定供试基因介导植株抵御干旱逆境的能力；采用高通量RNA-Seq和iTRAQ技术，解析重要TaNF-YB基因特异调控的转录谱、蛋白谱、基因网络和生化代谢途径。通过研究系统阐明小麦NF-YB型转录因子基因介导植株抵御干旱逆境的功能和分子机制。

Through transcriptionally regulation of downstream stress-responsive genes, NF-Y type transcription factors play critical roles in mediating plant drought stress tolerance. In this project, based on our previous research results and using ten NF-YB transcription factor encoding genes of wheat (TaNF-YB1-TaNF-YB10) as basis, we will investigate the expression paterns of these tested genes in responding drought stress by using qRT-PCR, detect the distribution pattern of the fusion of each NF-YB with reporter protein green fluorescent protein (GFP) by which to understand the subcellular localizations of the NF-YB subunits, and identify distinct wheat NF-YC and NF-YA subunits that interact with the tested TaNF-YB transcription factors by performing yeast two hybridization (Y-2H) and bimolecular fluorescence complementation (BiFC) analyses, by which to elucidate the interaction models among these TaNF-YBs, TaNF-YCs and TaNF-YAs. In addition, we will identify the function of the tested wheat NF-YB encoding genes in mediating plant drought stress tolerance based on gene overexpression and RNAi methods, dissect the transcriptome, proteomics, gene network and corresponding biochemical pathway modulated by important wheat NF-YB genes by RNA-seq and iTRAQ approaches. Our current project can elucidate the functions and the related molecular mechanisms of the wheat NF-YB family members in mediating the plant drought stress tolerance.