近年来，包括"安徽牛肉膏"肉制品掺假事件在内的食品质量与安全事件频发，而我国相应的检测技术、标准较为落后，造成了较大安全隐患。特别是针对肉类等动物源性食品掺假行为尚无成熟的鉴别技术可用，难点在于无法区别"样品污染"与"恶意添加"行为，更无法区分杂碎成分。本团队提出的"物种特异性、通用性引物/探针联用测定样品中动物源性成分百分比含量的RT-PCR技术"有望突破该难点，目前已取得一定工作基础。拟围绕遇到的诸如引物/探针特性调控、双重RT-PCR竞争性反应动力学、DNA模板受热断裂规律、置信区间设置等关键问题，应用RT-PCR、cDNA微阵列、热加工、perl语言编程、数学建模等多学科交叉技术开展相关技术及其基础理论研究，建立起成熟的适用于掺假鉴别，基于RT-PCR的物种特异性DNA和组织特异mRNA定量检测技术及其配套的引物/探针设计技术体系，为监管部门提供技术支撑和相关技术研发提供理论参考。

The incidents of meat species adulteration in meat products, such as the "beef extract in Anhui Province", frequently happened in recent years. The outdated detection technologies and standards have caused large hidden safty troubles in our country. Especialy for animal-derived food, there is no mature detection method currently. The difficulty of analytical methods of detecting meat species in animal-derived food lies to how to make a distinction between "sample contamination" and "malicious adulteration". The "species-specific combined with universal primers/probes" RT-PCR method which was used to determine the percentage content of animal-derived components developed by our research group is expected to exceed the difficulty and some research foundation has been made. Relevant technologies and its basic theoretical researches will be carried out around a series of key issues, such as properties control of prmers/probes, competitive reaction kinetics of duplex RT-PCR, the principles of DNA template heated fracture and confidence interval setting and so on by RT-PCR, cDNA array, thermal processing, perl programming, mathematical modeling technologies. The mature RT-PCR-based species-specific DNA and tissue-specific mRNA quantitative detection technology and associated primer/probe design technology system for adulteration identification will be established in order to provide technical supports and theoretical references for the regulatory authorities.