DNA 作为可精确调控的反应基元被用于构建分子机器及组装，并广泛应用于 DNA 分子计算、疾病诊治和生物传感等研究领域。在 DNA 组装研究领域中，基于“DNA tile”的组装引起人们很大兴趣。通过设计“DNA tile”边缘的接枝 DNA 链序列，能够实现 DNA tile 的可控链接和组装。在此申请中，我们拟构建基于“DNA tile”的组装基元，边缘接枝 DNA 单链长度和序列可以用于调控“DNA tile”基元间的“键接”方式和强度。借助高分子化学科学领域的聚合反应概念，结合 DNA 分子机器驱动“DNA tile”组装，实现基于“DNA tile”基元可控组装和调控。此处由 DNA 链构建的 “DNA tile”组装基元具有如下几个优势：1） 结构更具有可设计性，接枝在DNA tile 侧端的DNA 单链位置寻址；2）能够形成一维线性和二维片状结构。

With the programmable behaviors in building molecular machine and realization of self-assembly, DNA molecule has found broaden applications in the areas of DNA molecular computation, diagnosis of disease and biosensors. Currently, a strategy in which the DNA tiles are designed and built as an assembly unit ignites great interesting to the ones working in the fields. In this proposal, we suggest a strategy to utilize the DNA tile for building an assembly unit, on the side of which graft a multiple single DNA chains that will play role a “bond” as polymerizing with other “DNA tiles”. The bonding strength between tiles is able to be modulated by the length of these grafted chains. Furthermore, with the help of designed DNA molecular machine, the controllable polymerization of the DNA tiles is realized. It has several advantages with this particular strategy, 1. deignable to the structures of the DNA tiles and the positions of DNA chains grafted on the side of the tile; 2. able to form some linear chain or two-dimensional structures via polymerization.