颞下颌关节髁突缺损修复是临床中的一大挑战，因其软骨再生能力弱，新生软骨与软骨下骨间无法紧密结合而发生剥脱。本项目基于骨-软骨界面结构的理论依据，在前期工作的基础上，提出利用高性能PEGS/MBG双相支架诱导内源性BMSC进行髁突骨/软骨一体化修复：采用聚乙二醇-聚癸二酸甘油酯（PEGS）构建并优化新型粘弹性软骨诱导支架，并复合具有优良成骨诱导性的介孔生物玻璃（MBG）构建双相支架；设计“核壳”结构梯度粘弹性的PEGS支架，其内核高交联度提供高弹性力学强度，外壳低交联度提供粘弹性的软骨诱导微环境；引入3D打印技术构建针对颞下颌关节的精细结构。课题将从分子生物学至体内缺损修复开展系统的研究，研发兼具力学强度、骨/软骨诱导性能和精细结构的高活性骨/软骨一体化修复支架，阐明双相支架诱导内源性BMSC进行髁突软骨/骨缺损一体化修复中再生过程的机制，为未来髁突缺损修复提供新的实验基础和理论依据。

Clinically mandibular condylar defect repair often fails due to the insufficient integration of regenerated cartilage and subchondral bone. Based on the theoretical basis of the bone-cartilage interface structure, the idea of utilizing high-performance bilayer scaffolds to induce endogenous BMSC for integrated osteochondral repair was proposed in this project. A novel viscoelastic cartilage-inducing scaffold based on copolymer of poly(ethylene glycol) and poly(glycerol sebacate) (PEGS) was constructed and optimized. Biphasic scaffolds were constructed by compounding PEGS with well-known mesoporous bioactive glass (MBG) for the integrated repair of compound osteochondral condylar defect . Specifically for mandibular condylar defect, a "core-shell" gradient viscoelastic PEGS scaffold was designed, with its high cross-linking degree of the core providing high elastic mechanical property, and the low cross-linking degree of the shell provided a viscoelastic cartilage-inducing microenvironment. The fine structure of biphasic PEGS/MBG scaffold was constructed by 3D printing technology. Therefore, we shall carry out a systematic and comprehensive study from molecular biology to in vivo defect repair, clarifying the mechanism of biphasic scaffolds inducing endogenous BMSC to integratedly regenerated condyle, and develop highly active osteochondral integrated repairing scaffolds, providing new concept, experimental basis and theoretical support for future research of condylar defect repair.