基于前期研究中用含有相关胞内酶的细胞直接替代游离的NAD(P)H依赖型氧化还原酶可以检测该酶活性等系列新发现，以NAD(P)H依赖型甲酸脱氢酶、亮氨酸脱氢酶、甘油脱氢酶和/或醇脱氢酶；FAD依赖型胆固醇氧化酶（参照物）等各具代表性的氧化还原酶为研究对象，应用扫描隧道显微镜断裂连接（STM-BJ）系统考察各单分子酶结合辅酶、催化底物过程的实时导电性，并依此验证所提出的NAD(P)H依赖型氧化还原酶催化新机理的合理性。研究单分子NAD(P)H依赖型氧化还原酶及其与辅酶结合和催化底物反应的导电性属于原始创新，可望创立酶电子工程学，拓展生命活动中的酶学研究和改善酶工程领域的技术开发；所提出的NAD(P)H依赖型氧化还原酶催化新机理既是对沿袭近70年的传统机理的革新，又可望更新传统的全细胞发酵、多酶耦联催化、酶活性检测、生物传感器等技术，故本课题研究不仅具有重大的科学意义而且具有广阔的应用前景。

Based on the previous study, a series of new discoveries such as NAD(P)H-dependent oxidoreductase and brightening can be detected by directly replacing the free NAD(P)H-dependent oxidoreductase with cells containing related intracellular enzymes. The representative oxidoreductases such as formate dehydrogenase, leucine dehydrogenase, glycerol dehydrogenase and/or alcohol dehydrogenase, and FAD-dependent cholesterol oxidase (reference) are used as research objects, and scanning tunneling microscopy break junction(STM-BJ) is used for fracture connection systematically investigated the real-time conductivity of each single molecule enzyme in combination with coenzyme and catalytic substrate, and verified the rationality of the proposed new mechanism of NAD(P)H-dependent oxidoreductase catalysis. The study of single-molecule NAD(P)H-dependent oxidoreductase and its conductivity in combination with coenzyme and catalytic substrate is an original innovation. It is expected to create enzyme electronic engineering, expand enzymatic research in life activities and improve the development of enzyme engineering and technology; the proposed new mechanism of NAD(P)H-dependent oxidoreductase catalysis is not only the innovation of the traditional mechanism of the past 70 years, but also the renewal of traditional whole-cell fermentation, multi-enzyme coupling catalysis, Enzyme activity detection, biosensor and other technologies, so the research of this subject not only has great scientific significance but also has broad application prospects.