植物内源激素在种子发育过程中扮演着重要角色，理解其对种胚油脂积累与消减的调控作用，是创制高含油量育种材料的必要前提。我们实验室前期的研究表明：TT2调控类黄酮的合成，而类黄酮抑制油脂积累；TT2基因丧失功能导致种子含油量提高。初步实验数据表明，种胚内类黄酮影响生长素运输蛋白的分布与表达，从而与种胚局部生长素浓度、油脂合成的调控相关。然而，这一生理过程的分子机制仍不明晰。在这项研究中，我们旨在探明：（1）类黄酮对种胚生长素运输蛋白（PIN）的分布及种胚形态的影响；（2）种胚局部生长素浓度对调控种子发育关键转录因子、脂肪酸合成途径关键基因、油脂消减基因表达的影响；（3）种胚生长素浓度对种子成熟周期、含油量与脂肪酸组分、种子解剖结构、粒重等的影响。进一步厘清类黄酮物质、生长素、种子发育关键转录因子、种子油脂消减基因（SFAR）之间的互作关系，从而为分子育种提供理论依据并创制基础育种材料。

Plant hormones play important role in seed development. To understand how they regulate lipid accumulation and/or decomposition in seeds is a prerequisite of breeding programs towards high-oil-content seeds. Previous studies in our lab indicate that Transparent Testa2 (TT2) regulates the biosynthesis of flavonoids, and flavonoids not only inhibit lipid accumulation but also promotes lipid decomposition. The loss of TT2 function would eventually result in high-oil-content seeds. Our preliminary data also shows that embryonic flavonoid distribution affects expression of auxin transporters such as PIN4, and thus, represses lipid biosynthesis. However, the mechanism underlying this observation is not clear. In this proposed research, we plan to investigate (1) the effect of flavonoids on expression of PINs and embryo-shape formation; (2) the effect of auxin localization on expression of the key transcriptional regulators and important genes for lipid biosynthesis; (3) the effect of auxin on length of seed maturation process, seed oil content and composition, seed weight and morphological structure. The goal of this proposal is the understanding of relationships among flavonoids, auxin, and transcriptional factors for seed development, seed lipid accumulation/decomposition, the knowledge on which molecular breeding and gene manipulation base.