成年皮肤的无疤愈合或完美重建一直是科学家们探索伤口修复的终极目标。基于细胞外基质（Extracellular Matrix. ECM）形成的壁龛（Niche）对间充质干细胞（Mesenchymal Stem Cell, MSC）的重要影响，本项目拟深入探讨MSC在特异性的ECM生物机械硬度壁龛微环境下，对成年皮肤全皮层损伤修复的作用机理。采用双光子荧光(Two-Photon Fluorescence, TPF)和二次谐波（Second-Harmonic Genenation, SHG）非线性光学显微成像技术，3D动态、活体无损监测伤口修复过程中MSC的活动；定量分析对新生真皮层组织中胶原纤维的沉积、重构的影响；并利用生物信号通路探索对疤痕形成起关键影响的成肌纤维细胞（Myofibroblast）的调控。项目将为揭示皮肤潜在再生通路，发展“完美重建”受损成年皮肤的方法提供机理性研究基础。

The scarless healing or perfect reconstruction of adult skin is always the ultimate exploration target of scientists for wound repair. On the basis of the great influence of "niche" formed by extracellular matrix (ECM) on mesenchymal stem cells (MSCs), this project aims to look insight into the mechanism of microenvironment of ECM with specific biomechanical stiffness on full-thickness cutaneous wound repair of adults..Multiphoton microscopy of the two-photon fluorescence (TPF) and second-harmonic generation (SHG) are adopted for 3D dynamically monitoring the activities of MSC, quantitatively analyzing the impact on collagen deposition and reorganization in the newly formed dermal tissues during repair process. Also, by using biological signalling pathway, the regulation on myofibroblast, an important effector in scar formation is explored..This project will provide a foundation for mechanism revealing of potential pathway of skin regeneration and developing a "perfect reconstruction"method for injured adult skin.