蛋白效应子诱导植物免疫、提高抗病性是病害生物防治的新策略，植物受体对蛋白效应子的识别是揭示植物免疫抗性机制的重要起始点。MoHrip1是本项目申请团队从稻瘟菌(Magnaporthe oryzae)中分离的一种分泌型蛋白效应子，具有诱导水稻抗稻瘟病的功能，相关研究已申请国家发明专利（CN201210035653.4）。前期以MoHirp1为诱饵蛋白，用酵母双杂交技术筛选出18个候选互作蛋白，本项目拟在此基础上，用双分子荧光互补技术（BiFC）、GST pull-down和等温滴定微量热法（ITC）等技术验证MoHrip1诱导水稻抗性的蛋白受体；通过T-DNA插入突变、microRNA沉默以及水稻全基因组表达谱分析，在分子遗传水平上研究蛋白受体在MoHrip1诱导水稻抗病中的功能；为阐明MoHrip1诱导水稻抗病的分子机制提供理论基础。

MoHrip1，a protein elicitor isolated by our research team from Magnaporthe oryzae,could induce systemic acquired resistance to rice blast in rice.The research results have been applied for a national invention patent(CN201210035653.4). Plant recognition receptor of elicitor is the key point to reveal the immune mechanism of resistance in plants,but the rice receptor of MoHrip1 have not been reported so far.Now,we had screened 18 candidate interacting proteins by yeast two-hybrid system.We will verify these protein receptors with bimolecular fluorescence complementation (BiFC), GST pull-down and isothermal titration calorimetry (ITC) in vitro and in vivo.To study the function at a genetic level of the protein receptors, rice T-DNA insertion mutants, microRNA silencing protein interaction gene and rice whole-genome expression microarrays will be used with the gene mapping differences of gene silencing and over-expression.This research will provide a theoretical basis for understanding the molecular mechanism of rice disease resistance induced by MoHrip1.