激发子能诱导植物免疫反应，但对其诱导机制尚缺乏全面精确的认识。植物细胞受体对激发子识别是植物免疫防御反应的第一步，鉴定激发子的识别受体是明确其诱导机制的关键所在。本项目申请团队从极细链格孢菌中筛选获得蛋白激发子PeaT1，能诱导水稻免疫抗性，提高抗逆能力。并从水稻中筛选到PeaT1的互作蛋白肌醇加氧酶，克隆肌醇加氧酶（OsMIOX）基因并通过酵母双杂交验证能与PeaT1互作。本项目在此基础上，利用体内与体外互作验证方法结合验证PeaT1与候选受体肌醇加氧酶的互作；利用CRISPR-CAS9基因组定点编辑技术，获得候选受体的水稻突变体植株，通过候选受体基因定点剪切和修复等技术分析相关基因的表达差异与抗性的变化，结合基因过表达技术验证激发子的受体与抗逆关键基因的关系，阐明抗病关键基因在抗逆过程中的机制，为揭示链格孢激发子蛋白诱导免疫抗性与抗逆机理研究提供依据。

Protein elicitor can induce an immune response in plant, but the inducing mechanism is not comprehensive accurate understanding. The first step of plant immune defense reaction was the recognition between plant cell receptors and elicitor,this is also the key point to understand the induction mechanism. Protein elicitor PeaT1 was isolated from Alternaria tenuissima in our lab, it can induce the defense reaction in rice, improve its ability to resist against adverse reaction. Our preliminary work show that we had screened protein OsMIOX which can interact with elicitor from rice. The OsMIOX can interact with the PeaT1 by yeast two-hybrid system. In this project, pull down, bimolecular fluorescence complementation (BiFC)were applied to test the interaction with PeaT1 in vitro and in vivo; subsequently, Got the rice mutant of the candidate receptor Applied by the technology of CRISPR-CAS9,then, analyzed the change of defense reaction,the difference express of relative genes, combined with the over-expresson to identify the relationship of receptor and key genes for disease-resistant. expounded the function of key genes in the progress of plant defense reaction.This research will provide theoretic foundation to understand the molecular mechanism of PeaT1 induced defense responses in plants.