聚ADP核糖聚合酶1（PARP-1）参与猪卵巢卵泡闭锁过程中颗粒细胞自噬的分子机制研究

聚ADP核糖聚合酶(poly ADP-ribose polymerase, PARP)是一类NAD+依赖性且存在于大多数真核细胞内的蛋白质翻译后修饰酶。作为该家族的主要成员，PARP-1在多种生理或病理过程中起着至关重要的作用。我们早先的研究已经证实PARP-1参与了猪卵泡闭锁过程，但PARP-1在卵泡闭锁过程中确切的作用机制尚不明确。PARP-1与细胞自噬相关蛋白存在相互作用，我们最近的研究表明，猪卵巢颗粒细胞凋亡过程同时伴随着细胞自噬，但PARP-1在猪卵巢颗粒细胞自噬过程中的作用尚不清楚。本课题拟结合猪卵泡闭锁模型和颗粒细胞凋亡模型从在体-细胞-分子水平探究PARP-1参与猪卵巢卵泡闭锁过程中颗粒细胞自噬的分子机制。

PARP-1 is a founding member of poly ADP-ribose polymerase (PARP) families, which is a (NAD+)-dependent enzyme and plays vital roles during a variety of physiological and pathological processes. Our previous study demonstrated that PARP-1 was strongly implicated in porcine follicular atresia, however, its role in the process of follicular development and atresia was not yet completely defined. PARP-1 is involved in autophagy. Our recent data indicated that porcine granulosa cell apoptosis process was accompanied by autophagy. However, the role of PARP-1 during the process of porcine ovarian granulosa cell autophagy is not clear. In present project, we combined the in-vitro and in-vivo models to investigate roles of PARP-1 in granulosa cell autophagy during the process of porcine follicular atresia from In-vivo-Cell-Molecular levels.