阿尔茨海默病（AD）是一种成因复杂的神经退行性疾病。课题组前期研究表明，补肾益智方（BSYZ）可改善AD多种动物模型的学习记忆能力，其机制可能是通过SIRT1-ER stress信号通路，拮抗Aβ毒性诱导的ER stress反应性神经元凋亡。BSYZ复方能明显提高蛇床子单味药、蛇床子素的生物利用度。而细胞色素P450（CYP450）参与内源性物质和包括药物、环境化合物在内的外源性物质的代谢。CYP3A是CYP酶家族最主要的酶系之一，也是介导蛇床子素体外代谢的重要介质。因此，课题组拟从肠道对蛇床子素代谢的角度出发，探讨BSYZ复方中的有效成分蛇床子素的代谢过程是如何受到复方的影响，以及复方中哪些单体成分可抑制CYP3A4酶活性，从而提高了复方中的蛇床子素生物利用度，为BSYZ治疗AD的临床应用及新药开发提供实验依据。

Alzheimer's disease (AD) is one of neurodegenerative diseases with complex causes. In our recent study, Bushen-Yizhi formula (BSYZ) could improve the learning and memory capability in several kinds of AD animal models, whose mechanism may be that neuron apoptosis caused by Aβ toxicity could be reduced by ER stress reactivity through the SIRT1 - ER stress signaling pathways. BSYZ formula could significantly improve the bioavailability of Fructus Cnidii and osthol. Cytochrome P450 (CYP450) is involved in the metabolism of endogenous substances and exogenous substances, including drugs and environmental compounds. CYP3A is one of the most important enzymes in the CYP family, and is also an important medium for mediating the metabolism of osthol. Therefore, we will focus on the intestinal metabolism to osthol, to investigate how osthol is influenced by the effective components of BSYZ formula, and which of those components can inhibit CYP3A4 enzyme activity, then improve the bioavailability of osthol. The result will provide beneficial evidences for clinical application and new drug development of BSYZ on treating Alzheimer's disease.