Dmrt1是雄性性别分化和性别决定的关键基因，主要表达于脊椎动物精巢支持细胞中。然而，在石斑鱼中Dmrt1特异性的表达于精原细胞和精母细胞中。Rec8是精子发生过程必不可少的基因，对生物开启减数分裂至关重要。在前期的研究中，我们发现Dmrt1能够促进斜带石斑鱼Rec8的表达，并且在rec8基因启动子上存在三个Dmrt1结合位点，提示生殖细胞中的Dmrt1可能对rec8有直接的调控作用。为此，本项目拟通过基因表达分析、染色质免疫共沉淀、凝胶阻滞实验、启动子缺失突变、双荧光素酶报告系统和在体离体实验探究Dmrt1对Rec8的调控机制。此外，通过单独和同时降低Dmrt1、Rec8表达分析二者对斜带石斑鱼精子发生的作用机制。理论上，有助于解析Dmrt1通过Rec8调控石斑鱼精子发生的作用和机制；应用上，为解决近年来在育种过程中出现的雄性石斑鱼产精难的问题提供思路。

Dmrt1 is key gene for male sex differentiation and sex determination, it is mainly expressed in sertoli cells, but in grouper, the Dmrt1 expression in spermatogonium and spermatocyte. Rec8 is an essential gene in the process of spermatogenesis, and it is important to initiate meiosis. In previous studies , we found Dmrt1 can promote Rec8 expression in Orange-spotted grouper, and three Dmrt1 binding sites were found on the rec8 gene promoter, suggests that Dmrt1 in the germ cells of grouper may regulate rec8 gene. In this project, we using gene expression analysis, chromatin immunoprecipitation, electrophoretic mobility shift assay, promoter deletion mutation, dual-luciferase reporter gene assay, in vivo and in vitro experiments to determine the transcriptional regulation mechanism of Dmrt1 and Rec8. In addition, the effect of Dmrt1 and Rec8 on spermatogenesis was observed by single and simultaneously knock down these two genes. In terms of theory, we revealed the effect and mechanism of Dmrt1 regulation spermatogenesis in orange-spotted grouper by Rec8. On the application, this research may help to solve the problem of male grouper spermatogenetic failure in recent years.