神经活动荧光成像技术和光遗传技术在对神经环路研究中已经取得重要研究成果，但仍不能完全满足神经环路功能研究的需求，主要表现为：活体实验中单纯的荧光成像，神经活动相关荧光信号会受到内源生色团浓度变化的干扰；传统神经元光调控方法只能进行对神经环路进行单个空间点的调控，无法实现神经环路多时空模式的调控；目前尚无能够对清醒活动中实验对象进行神经环路光调控同时进行神经活动荧光成像的方法。因此，本项目拟发展神经环路的多模态光学成像方法和荧光信号解析的矫正方法，改进空间光调制的神经元光调控技术，并解决两种方法同时用于清醒活动实验对象的关键问题，研究用于清醒动物的光纤型神经环路光调控与荧光成像一体化系统。

Fluorescence imaging and optogenetics have been playing important roles in neural circuits research. However, they still can not fully meet the needs of the studies on the function of neural circuits. First, the interference of changes in intrinsic chromophore would be involved in the common used in-vivo fluorescence imaging. Second, traditional photostimulation can only apply the stimulation at single point which can not provide multiple spatial pattern of stimulation. Last, there is no experimental setup which can provide simultaneous photostimulation and fluorescence imaging on freely moving animal yet. In this project we plan to develop the optical imaging techniques that combine the fiberic multi-modal optical imaging with the DMD based multi-pattern photostimulation so that the fluoresence imaging and multi-pattern photostimulation can be performed on freely moving animals simultaneously.