血小板的粘附和聚集是血栓形成的始动因素，抗血小板药物是血栓病预防和治疗的“基石”。然而，同一种抗血小板药物对不同患者所产生的抗血小板效应差异很大，因此，亟需建立可靠的血小板功能检测新方法以指导临床用药。由于血小板极易受外界刺激而活化，本项目拟建立集确定性侧向位移(DLD)、膜分离及离子浓差极化（ICP）三种微流控芯片于一体的血小板功能检测新方法，通过微泵精准控制生理水平的流动剪切率，连续实现全血中血小板的粘附、聚集功能检测，游离血小板和血浆的分离，血小板膜上P选择素（CD62p）和血浆β-血小板球蛋白（β-TG）的水平测定。整个动态检测过程采用同一荧光倒置显微镜在线连续完成，既可减少外界刺激对血小板的激活，准确再现体内血液循环中血小板的功能状态，又可同时检测血小板活化标志物的水平和最终的聚集状态，为血栓性疾病的临床诊断及抗血小板个体化治疗决策提供更有力的依据。

Platelet adhesion and aggregation are essential for thrombosis. Antiplatelet drugs are the cornerstone of prevention and treatment of thrombosis. However, the antiplatelet effect of the same antiplatelet drug has great individual differences, therefore it is urgent to develop a reliable new method of platelet function test to guide clinical medication. Platelet is easily activated because of its high susceptibility to external stimulation, the purpose of this project is to develop a new method of multifunctional platelet test based on the deterministic lateral displacement (DLD), membrane separation and ionic concentration polarization (ICP) microfluidic chips, which can be used to continuously detect the adhesion and aggregation of platelets, separate free platelet and plasma from whole blood under the flow shear rate at the physiological level controlled by micropump and determine the levels of p-selection (CD62p) of platelet and β-TG of plasma. The determination of the whole dynamic process will be completed online using an inverted fluorescence microscope, which can reduce the platelet activation induced by external stimulation to accurately represent the platelet function status in blood circulation as well as detect the level of platelet activation markers and the final state of aggregation to provide more powerful evidences for clinical diagnosis of thrombotic diseases and individualized antiplatelet treatment.