电针可以改善手术创伤引起的下丘脑-垂体-肾上腺轴功能异常，但其神经科学机制需要进一步阐明。 本课题首先从新近发现的应激过程的“触发器”SCGN入手，探讨下丘脑 SCGN在电针调节围手术期大鼠HPA轴异常功能中的作用。下丘脑NMDA受体及其激活的胞内信号通路是否参与电针调节围手术期大鼠HPA轴功能异常还不清楚，其是否通过影响SCGN的合成和表达，参与电针调节围手术期HPA轴功能异常机制尚未见报道。电针是否通过调节靶向NMDA受体的miRNAs参与对围手术期大鼠HPA轴异常功能的调节值得深入研究。本课题拟在前期研究的基础上，采用大鼠部分肝切术模型，从整体、细胞和分子水平来探讨SCGN、NMDA受体及其胞内信号通路中的关键分子以及NMDA受体相关的miRNA参与电针调节围手术期大鼠HPA轴功能异常的机理，为围手术期应激发生和缓解机制提供实验和理论依据；为临床EA推广提供理论依据。

Electroacupuncture (EA) can improve the dysfunction of hypothalamus-pituitary -adrenal axis caused by surgical trauma, while its neurobiological mechanisms remain to be clarified. In this study, we will focus on the newly discovered key molecule secretagogin (SCGN), the trigger of stress process, which is the key molecule regulates the release of CRH. We will explore the role of SCGN in hypothalamic PVN in EA modulating the abnormal function of HPA axis induced by acute surgical trauma, and further elaborate the mechanism of EA. It has been studied for decades on hypothalamic NMDA receptor regulation of CRF synthesis and release, whether hypothalamic NMDA receptors and its activated intracellular signaling pathways participate in the EA regulation of HPA axis dysfunction in rats during perioperative period is unclear. Whether they participate in EA regulated the dysfunction of HPA axis during perioperative period through affecting the synthesis and expression of SCGN has not been reported. Whether hypothalamic NMDA receptor-related microRNAs participate in the EA regulation of HPA axis dysfunction in rats during perioperative period deserves further study. On the basis of the previous studies, we will explore the mechanism of SCGN, NMDA receptors and their key molecules in intracellular signaling pathway, and microRNAs targeted NMDA receptor participating in the EA regulation of HPA axis dysfunction in rats from cellular and molecular levels on partial hepatectomy model, so as to provide experimental and theoretical basis on the EA alleviating stress response during perioperation period, and provide a theoretical basis on EA popularization in clinical practice.