血管低反应性和血管渗漏为休克等临床重症多脏器功能损害的关键因素，现有临床治疗措施难以协同解决其救治问题。周细胞为血管壁周围一种多功能细胞，在多种疾病中发挥重要作用。基于本实验室前期和预实验结果，推测周细胞对严重创伤休克血管反应性和血管通透性有协同调控和保护作用。为此，本项目拟采用严重创伤休克大鼠模型、周细胞敲减模型和周细胞、VEC、VSMC 3D及双室培养模型，研究周细胞对严重创伤休克血管反应性和血管通透性的协同调控和保护作用机制，重点研究周细胞分泌microvesicle携带活性物质，以及通过与VSMC和VEC间的缝隙连接和粘附连接协同调控血管反应性和血管通透性的作用及机制。阐明这一问题可从新的角度阐明休克等临床重症血管低反应性和血管渗漏的机制，为其治疗提供新的治疗靶点。

Vascular hyporeactivity and leakage are the key pathological courses that many critical illnesses such as severe trauma, shock and sepsis induce multiple organ dysfunction. There are no effective measures that can synergistically solve the treatment of vascular hyporeactivity and vascular leakage in clinic at present. Pericyte, a kind of multiple functional cell located around blood vessels, plays important roles in many diseases. Based on our previous and pilot studies, we hypothesize that pericytes may play synergistic regulation in vascular contractile/relaxing response and barrier function via synergistically regulating vascular smooth cell (VSMC) and vascular endothelial cell (VEC). In order to testify this hypothesis, using traumatic shock and pericyte knockdown animal model and three dimension and two compartment cultured pericytes, VECs and VSMCs, and cell biological, molecular and biochemical techniques, we tend to investigate: (1) whether pericytes can synergistically regulate VECs and VSMCs, playing pivotal role in the regulation of vascular contractile response and barrier function following shock; (2) except for secretion of cytokines and mediators, whether pericytes can release extravascular vesicle such as microparticle carrying active substances and MiRNAs or via direct communication with VECs and VSMCs to regulate VECs and VSMCs. Elucidating this issue and precise regulatory mechanisms would further interpret the mechanism of vascular hyporeactivty and vascular leakage following critical illnesses and understand the role of intercellular communication in these diseases in a new angle, and to provide the new direction and targets for prevention and treatment of these critical illnesses in clinic.