由于大水面水产养殖不可避免地给水环境造成污染，因此，将会受到越来越严格的管控。集约化养殖将成为我国水产养殖发展的重要方向。集约化养殖迫切需要我们培育出耐低氧鱼类新品种。作为我国淡水水产养殖的重要养殖对象，鲫鱼具有极度耐低氧的优良性状。本项目拟以耐低氧的鲫鱼为主要研究对象，并以对低氧敏感的团头鲂作为对照物种，以斑马鱼作为模式鱼类，利用分子生物学、遗传学和基因编辑等技术，筛选和鉴定与鲫耐低氧性状相关的基因，构建鲫耐低氧的基因调控网络，解析鲫耐低氧相关基因的生物学功能及其发挥生物学功能的分子机制，阐明鲫耐低氧优良性状的遗传基础，挖掘鱼类耐低氧优良基因，为培育耐低氧鱼类新品种奠定基础。

It will be strictly controlled for the aquaculture of large water surface because it causes water pollution inevitably in our country. Intensive aquaculture will become a main direction of aquaculture in China, which requires us to obtain new species or strains with hypoxia tolerance. As one of main species in aquaculture of our country, Crucian carp has excellent economic characters in hypoxia tolerance. In this proposal, we propose to take advantage of Crucial carp as research materials, and use Bluntnose back bream as controls, as well as zebrafish as a model system. Firstly, we will use technologies including molecular biology, genetics and gene editing to screen and identify genes related to hypoxia tolerance in Crucian carp. Then, we will construct a regulatory network of these genes. Secondly, we will investigate the function of these genes and the underlying molecular mechanisms. Eventually, we will demonstrate the genetic basis of Crucian carp in hypoxia tolerance and figure out the genes related to excellent economic characters in fish. These results will build up the foundation for cultivating new species or strains of fish with hypoxia tolerance.