大豆抗原蛋白能够引起仔猪、犊牛等幼龄动物肠道损伤。本课题组前期研究表明，大豆抗原蛋白通过MAPK、NF-κB信号通路，引起肠道损伤，但是关于内质网应激/NLRP3炎症小体信号通路在仔猪肠道损伤中的作用，有待研究。本项目以“大豆抗原蛋白通过内质网应激/NLRP3炎症小体信号通路诱导仔猪肠道炎性损伤”为理论假设，通过大豆抗原蛋白体内饲喂和体外细胞培养试验，研究其对仔猪肠黏膜和细胞中内质网应激和NLRP3炎症小体相关蛋白及紧密连接蛋白表达、D-乳酸和炎性因子的含量、IELs亚群比例、肠黏膜及细胞超微结构变化的影响，以确证大豆抗原蛋白通过内质网应激/NLRP3炎症小体信号通路引起仔猪肠道炎症反应。本研究进一步揭示大豆抗原蛋白对仔肠道炎性损伤的分子机制，为弱化其损伤作用提供理论依据。

Soybean antigen protein can cause intestinal damage to piglets, calves and other young animals. Our previous research has demonstrated that soybean antigen protein caused intestinal damage via MAPK and NF-κB signaling pathways. However, the role of endoplasmic reticulum stress/NLRP3 inflammasome signaling pathway in intestinal damage of piglets remains unclear. In this study, we hypothesised that soybean antigen protein would induce intestinal inflammatory injury in piglets via endoplasmic reticulum stress/NLRP3 inflammasome signaling pathway. Soybean antigen protein in vivo feeding and in vitro cell-cultured experiments are conducted to study its effects to endoplasmic reticulum and NLRP3 inflammasome-associated proteins and tight junction proteins, D-lactic acid and inflammatory factors, and the ratio of IELs subgroups in intestinal mucosa and cells, the ultrastructural changes in cells and intestinal mucosa, which will clarify the endoplasmic reticulum stress/NLRP3 inflammasome signaling pathway of intestinal inflammatory injury induced by soybean antigen protein. This study will reveal molecular mechanism of soybean antigen protein on intestinal inflammatory injury of piglets, which provide a theoretical basis for weakening intestinal damage.