调控血管平滑肌细胞( VSMC )表型转换是防治PCI术后再狭窄的新策略, microRNA作为VSMC表型转换的调节因子，可通过转录后水平调控下游靶基因表达而影响VSMC表型转换。本组首先克隆的C2H2型锌指核转录因子ZFP580可调控内皮细胞及VSMC的增殖及迁移而影响血管重塑。本组新近实验证实: microRNA-206既可靶向调控ZFP580表达，又可促进VSMC从收缩型向合成型转换。本项目拟采用慢病毒microRNA-up及microRNA-down技术，应用原代VSMC体外实验、球囊损伤大鼠颈总动脉体内实验、紫外交联免疫沉淀结合高通量测序及生物信息学分析，在基因、细胞及动物整体水平上揭示microRNA-206靶向ZFP580调控球囊损伤后平滑肌细胞表型转换的分子机制，为研究和防治球囊扩张PCI术后再狭窄提供新的科学依据。

It is a new strategy to control the vascular smooth muscle cells( VSMC ) phenotype conversion in order to prevent restenosis after PCI. As the regulators of VSMC phenotypic conversion, MicroRNAs can influence the phenotype conversion of VSMC by down-regulating its downstream target genes expression at the post-transcriptional levels. ZFP580,a novel C2H2 type zinc finger transcription factor cloned by our lab, could modulate the vascular remodeling by means of regulating the proliferation and migration of endothelial cells and VSMC. It was newly confirmed by our lab that microRNA-206 can down-regulate the expression of ZFP580 and promote the conversion of VSMC from contractile to synthetic type. Our project will plan to use lenti virus vector to microRNA-up/ microRNA-down the microRNA-206 of primary VSMC in vitro and to microRNA-down the microRNA-206 in balloon injuried carotid artery of rats in vivo.Furthermore, both the crosslinking-immunprecipitation/high-throughput sequencing and the bioinformatics analysis will be applied to reveal the molecular mechanism of microRNA-206 targeting ZFP580 modulating smooth muscle cell's phenotype conversion after balloon injury. Our study will provide new scientific data to prevent restenosis after PCI.