细胞选择性梯度材料表面蛋白质粘附的时空演化及其对细胞迁移的影响

梯度生物材料可以提供一个非对称的化学物理环境，在血管、神经和骨-软骨等组织的再生中有着非常重要的作用。在血清介质或者体液环境下，其调控细胞的行为同样受到表面粘附的蛋白质的种类、数量及其随时间和空间位置变化的介导。本项目通过关联梯度生物材料，尤其是细胞选择性梯度生物材料表面的蛋白质粘附行为和细胞迁移行为，阐明梯度生物材料调控细胞迁移的内在机制，为先进生物材料的表面设计奠定理论依据。内容包括制备与表征能对内皮细胞和神经细胞选择性粘附进而调控其迁移速率和方向的梯度生物材料；研究多细胞环境下内皮细胞和神经细胞的粘附与迁移行为；阐明血清蛋白质在梯度生物材料表面粘附的种类、数量、构象取向、动力学和热力学的时空演化规律。最后，总结梯度生物材料表面细胞粘附、迁移－蛋白质粘附之间的规律和内在联系，建立梯度生物材料－蛋白质粘附－细胞迁移的相关模型。

The gradient biomaterials can offer an unsymmetrical niche in physicochemical properties, which is particularly important for the regeneration of blood vessels, nerves and osteochondral tissues. In the serum-containing mediums and body fluid, this type of biomaterials is similarly adsorbed with serum proteins of different species, amount, and confirmation along with the change of spatial position and evolution of time, and thereby mediates the cellular behaviors. In this project, the correlation between the protein adsorption behaviors and cell motility on the gradient biomaterials, especially the cell-selective gradient biomaterials shall be elucidated to clarify the intrinsic mechanism of cell migration on the gradient biomaterials, and thus providing theoretical bases for design of novel advanced biomaterials. Firstly, the gradient biomaterials that can selectively allow the attachment of endothelial cells (EC) and nerves cells (NC), and thereby guide their migration in terms of rate and direction shall be fabricated and characterized. Then, the competitive attachment and motility of the targeted cells (EC and NC) in the presence of other types of cells shall be studied. Next, the species, amount, confirmation, activity, and dynamics and thermodynamics of serum proteins adsorbed on the gradient biomaterials as well their evolution as a function of spatial position and time shall be clarified. Finally, the results and principles of the cell attachment and motility, and protein adsorption shall be analyzed and reasonably correlated to figure out the interaction model between the gradient biomaterials, protein adsorption, and cell migration.