基于线粒体氧化应激反应调控PGC-1α/ERRα1信号通路探讨健脾益肾强骨针法干预骨质疏松性髋骨骨折的分子机制

活性氧（ROS）诱导的氧化应激是骨质疏松症（OP）的重要发病机制，其中线粒体损伤引发的应激信号传导作用关键。前期研究证实健脾益肾强骨针法能通过同时提升股骨和股四头肌的力学性能而干预OP性髋骨骨折的发生，其中股四头肌抗外力能力提升发挥主要作用，研究还发现针刺不是通过改变股四头肌的宏观结构，而是通过改变微观结构达到功能的改善，骨骼肌线粒体可能是针刺的关键作用靶点。为进一步探索针刺是否主要通过骨骼肌细胞线粒体氧化应激引发PGC-1α/ERRα1信号通路而起到同时影响骨骼肌微结构和由成骨细胞/破骨细胞主导的骨代谢，拟以OP模型大鼠施以健脾益肾强骨针法，采用Western Blot、RT-qPCR等实验技术，通过检测骨骼肌线粒体呼吸复合物和PGC-1α/ERRα1通路中相关蛋白的表达，揭示健脾益肾强骨针法干预OP性髋骨骨折的分子机制，形成力学性能--形态学--细胞学--分子学完整的针刺效应作用途径。

Abstract: Oxidative stress induced by ROS is an important pathogenesis of osteoporosis (OP), in which stress signaling caused by mitochondrial damage is critical. Previous studies have confirmed that Spleen- Invigorating，Kidney- Tonifying and Bone- Reinforcing Acupuncture Method can interfere with the occurrence of OP hip fractures by simultaneously improving the mechanical properties of the femur and quadriceps, and the improvement of the quadriceps' ability to resist external forces plays a major role. The study also found Acupuncture does not change the macrostructure (wet weight, volume) of the quadriceps muscles, but improves the function by changing the microstructure (muscle cells). Among them, skeletal muscle mitochondria may be the key target of acupuncture. In order to further explore whether acupuncture mainly induces PGC-1α / ERRα1 signaling pathway through mitochondrial oxidative stress of skeletal muscle cells, it affects both skeletal muscle microstructure and bone metabolism dominated by osteoblasts / osteoclasts, We planned to castrate female rats, apply Zu Sanli, Shen Shu, Da Zhu as the main points, Spleen- Invigorating，Kidney- Tonifying and Bone- Reinforcing Acupuncture Method with twisting and tonifying method, use Western Blot, RT-qPCR and other experimental techniques by detecting the expression of skeletal muscle mitochondrial respiratory complex and related proteins in PGC-1α / ERRα1 pathway. The molecular mechanism of Spleen-Invigorating,Kidney-Tonifying and Bone-Reinforcing Acupuncture Method intervention for OP hip fractures was revealed to form: mechanical properties—morphology—cells Science—molecular science complete acupuncture effect pathway.