在对梨花粉管质膜离子通道活性研究的基础上，综合应用膜片钳、激光共聚焦显微镜、免疫印记及荧光标记等研究手段和技术，从信号分子与信息传递、生化代谢及超微结构等层面深入研究梨花粉管质膜离子通道与其他信号分子对梨花粉萌发与生长的协同作用机制。主要内容包括梨花粉管质膜钙和钾通道基因的克隆；环核苷酸等信号分子对梨花粉管质膜钙通道活性的调控机制；梨花粉管胞内钙信号与花粉管细胞微丝骨架、囊泡运动等的关系；血红素等信号分子对花粉管钾通道活性的调控机制；亲和与不亲和S-RNase对花粉管钙和钾通道活性的调控特性，以及钙和钾通道的开关对自交不亲和反应引起的花粉管胞内自由钙浓度、微丝骨架、囊泡运动变化等的影响。这些问题的解决将进一步明确梨花粉管质膜离子通道的信号转导机制，填补该领域的一些理论和技术的空白，对于拓宽果树生殖生理与细胞生物学的研究方向，推动对果树学及细胞生物学学科交叉科学问题的探讨具有一定的意义。

Based on the study of pear pollen tube plasma membrane ion channels, we used multiple techniques including in patch clamp, laser confocal microscopy, immunoblotting and fluorescent labeling to study the collaborative regulation mechanism(s) of pear pollen tube plasma membrane ion channels with other signaling molecules in controlling pollen germination and pollen tube growth. The main research includes the pear pollen tube plasma membrane calcium and potassium channels gene cloning; the regulatory mechanism(s) of signaling molecules such as cyclic nucleotide on pear pollen tube plasma membrane calcium channel activity; the relationship between calcium signaling and actin cytoskeleton structure, vesicle movement in pear pollen tube; the regulatory mechanism(s) of signaling molecules such as heme on pear pollen tube plasma membrane potassium channel activity; the regulatory mechanism(s) of compatible and incompatible S-RNase on pear pollen tube plasma membrane calcium and potassium channels activity, as well as the effect of opening and closing of pear pollen tube plasma membrane calcium and potassium channels on the incompatible S-RNase-induced alterations of cytosolic calcium concentration, actin cytoskeleton structure and vesicle movement. These studies will help us further understanding of the signal transduction of pear pollen tube plasma membrane ion channels, and promote the interdisciplinary scientific problems research between pomology and cell biology.