cAMP/PKA- - ERK信号途径联动在单色光诱导肉鸡淋巴细胞增殖中的作用

我们前一个结题基金研究发现蓝色光能上调松果体和视网膜的视蛋白基因OPN4-1和CHK-1转录、AANAT酶（褪黑激素合成关键酶）的mRNA表达与褪黑激素分泌，促进脾脏淋巴细胞增殖，提高肉鸡免疫功能，但对褪黑激素在单色光诱导肉鸡淋巴细胞增殖中的作用机制仍不完全清楚。本课题设计将肉鸡分别接受红光（660 nm）、绿光（560 nm）和蓝光（480 nm）等不同单色光以及白光（400-700 nm，对照）的照射，探究单光色对肉鸡淋巴细胞褪黑激素受体表达的影响，褪黑激素在调控单光色诱导的肉鸡淋巴细胞增殖中的受体途径、膜-核受体信号通路、胞内信号cAMP依赖的PKA与ERK途径联动的作用。从细胞与分子水平上进一步阐明单色光诱导肉鸡淋巴细胞增殖机制：褪黑激素作用假说，有助于揭示环境因子（光色）影响机体免疫功能的内在联系规律，有望为合理利用光色提高家禽免疫力和克服大量使用抗生素的负面影响提供新的突破点。

Our previous studies have shown that blue monochromatic light controlled the transcription of opsin gen (OPN4-1 and CHK-1) and the expression of AANAT enzyme mRNA in pineal and retina, and affected the secretion of melatonin to promote spleen lymphocyte proliferation and immune function in broilers. However, the regulatory mechanism of melatonin on monochromatic light-induced lymphocyte proliferation is still not well established. In this study, broilers were reared under red (660nm), green (560nm) and blue (480nm) monochromatic lights and white light (400-700nm) as a control, respectively. The role of monochromatic light on expression of melatonin receptor in lymphocytes, and the physiological effects of melatonin receptors and crosstalk between cAMP-dependent PKA and ERK intracellular signaling pathways on the monochromatic light-induced lymphocyte proliferation were studied in vivo and vitro. In this way, the mechanism of monochromatic light-induced lymphocyte proliferation will be clarified at the cellular and molecular levels. The hypothesis of melatonin will be helpful to discover the inner relationship between the environment factor (such as light spectra) and the avian immune function. It may suggest a new viewpoint to enhance the avian immune function by using light spectra and help to deal with the problem of antibiotic abuse.