稻瘟病菌致病机制研究是稻瘟病防治的基础和植物病原真菌致病机理研究的模式。酵母Snf1激酶家族是适应葡萄糖胁迫下利用其他碳源（如蔗糖、乙醇、麦芽糖等）所必须的，是受葡萄糖阻遏效应基因的主要调控因子；Snf1激酶参与细胞减数分裂、产孢、老化、单倍体侵入性菌丝和二倍体假菌丝的生长等。分析发现，稻瘟病菌Snf1复合体组成以及上游激酶发生了变化；稻瘟病菌Snf1途径被打断，稻瘟病菌气生菌丝变的稀疏，产孢量显著下降，致病性完全消失。本研究，拟通过野生型、突变体及恢复子的分析，在生化代谢、营养利用、细胞结构、形态发育及致病过程等多个层面分析Snf1途径参与的生物学过程；结合基因表达与定位、调控与蛋白互作、表达谱分析等研究，明确Snf1途径的作用机制，分析Snf1复合体的组成以及上下游相关激酶的功能，确定Snf1家族在稻瘟病菌中的地位，为稻瘟病菌的致病机理研究奠定良好的基础。

Investigation of the pathogenic mechanisms in the rice blast fungus provides the basis for the prevention and treatment of the rice blast. It can also serve as a model for studying the mechanism of pathogenicity in other plant pathogenic fungi. The Snf1 family in yeast is essential for its adaption to the glucose stress and the utilization of other carbon sources under this condition, such as sucrose and maltose. This family is the major regulatory factor for the glucose repression genes. SNF1 also has roles in meiosis, sporulation, aging, haploid invasive growth and diploid pseudohyphal growth.In our research, variations were found in the components of the Snf1 complex and its upstream in the rice blast fungus. Disruption of the Snf1 pathway showed sparse hyphae, reduced conidiation and failure to penetration. In this study, we will analyze the cell structure, the morphogenesis and pathogenesis in the wild type, mutants and the complementation strains. The pathway where the Snf1 complex is involved in will be clarified. We will analyze the localization and regulation of the gene, express the gene and study protein interaction using the expressed protein. In the end, we will clarify the function of the Snf1 pathway and the composition of the Snf1 complex in M. oryzae. All these results will lay a good foundation for the pathogenesis study of M. oryzae.