稻瘟病菌是真菌与植物互作研究的模式。Cdk8为中心的激酶模块是中介体复合物中唯一一个具有激酶活性的模块，这个模块调控真核生物发育相关的信号通路。本项目，围绕Cdk8激酶模块，开展稻瘟病菌中介体复合物的研究。利用高通量基因敲除的方法，获得基因的缺失突变体，结合生理生化，细胞学等分析，阐明基因的生物学功能；结合表达谱，磷酸化组学和蛋白互作分析，明确激酶亚模块在稻瘟病菌生长发育及致病过程中的作用。此项目，是对稻瘟病菌中介体复合物的初步探索，将为从根本上防治稻瘟病提供理论依据和可能的新药物靶点。

Magnaporthe oryzae is a model system for understanding microbe-plant interactions. Cdk8 module is the sole kinase activity part of the mediator complex. It regulates signal pathways of development in the eukaryotic cells. In this project, we propose to investigate the functions of mediator complex in M. oryzae based on the Cdk8 module. Gene null mutants will be obtained using high-throughput knockout strategy. The phenotypes of the null mutants will be analyzed by physiology, biochemistry and cytology methods. The biological functions of every gene in Cdk8 module will be demonstrated in M. oryzae. In addition, we will depict the functions of Cdk8 module in the development and pathogenesis through the combined strategies integrating gene expression profiling, phosphoproteome and protein interaction. The proposed project is the primary exploration of the functions of the mediator complex in M. oryzae. It would supply the novel theory evidence and drug target to control the rice blast.