MicroRNAs in Cerebrospinal Fluid as Potential Biomarkers for Parkinson's Disease and Multiple System Atrophy.
MicroRNAs in Cerebrospinal Fluid as Potential Biomarkers for Parkinson's Disease and Multiple System Atrophy.
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DOI：
10.1007/s12035-016-0253-0
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发表时间：
2017-12
影响因子：
5.1
通讯作者：
Verbeek MM
中科院分区：
文献类型：
--
作者：
Marques TM;Kuiperij HB;Bruinsma IB;van Rumund A;Aerts MB;Esselink RAJ;Bloem BR;Verbeek MM
帕金森病（PD）和多系统萎缩症（MSA）均属于神经退行性运动障碍和α-突触核蛋白病，症状重叠，尤其是在疾病早期，但疾病进展和对多巴胺能治疗的反应不同。因此，具有特异性分类患者的生物标志物，其可以区分PD和MSA，将是非常有用的。微RNA（miRNAs）调节蛋白质翻译，并在生物体液（包括脑脊液（CSF））中观察到，因此可能具有作为疾病生物标志物的潜力。我们研究的目的是确定CSF中的miRNAs是否可用作PD或MSA的生物标志物。使用定量PCR（qPCR），我们评估了来自PD（n = 28）、MSA（n = 17）和非神经系统对照（n = 28）的CSF患者样品中10种miRNA的表达水平。我们鉴定了区分PD与对照的两种miRNA（miR-24和miR-205）和区分MSA与对照的四种miRNA（miR-19 a、miR-19 b、miR-24和miR-34 c）。miRNA的组合准确地将PD（曲线下面积（AUC）= 0.96）或MSA（AUC = 0.86）与对照区分开。在MSA中，我们还观察到miR-24和miR-148 b与小脑共济失调症状相关，表明这些miRNAs参与了MSA的小脑变性。我们的研究结果支持miRNA组作为运动障碍生物标志物的潜力，并可能为这些疾病相关的病理机制提供更多见解。
Parkinson’s disease (PD) and multiple system atrophy (MSA) are both part of the spectrum of neurodegenerative movement disorders and α-synucleinopathies with overlap of symptoms especially at early stages of the disease but with distinct disease progression and responses to dopaminergic treatment. Therefore, having biomarkers that specifically classify patients, which could discriminate PD from MSA, would be very useful. MicroRNAs (miRNAs) regulate protein translation and are observed in biological fluids, including cerebrospinal fluid (CSF), and may therefore have potential as biomarkers of disease. The aim of our study was to determine if miRNAs in CSF could be used as biomarkers for either PD or MSA. Using quantitative PCR (qPCR), we evaluated expression levels of 10 miRNAs in CSF patient samples from PD (n = 28), MSA (n = 17), and non-neurological controls (n = 28). We identified two miRNAs (miR-24 and miR-205) that distinguished PD from controls and four miRNAs that differentiated MSA from controls (miR-19a, miR-19b, miR-24, and miR-34c). Combinations of miRNAs accurately discriminated either PD (area under the curve (AUC) = 0.96) or MSA (AUC = 0.86) from controls. In MSA, we also observed that miR-24 and miR-148b correlated with cerebellar ataxia symptoms, suggesting that these miRNAs are involved in cerebellar degeneration in MSA. Our findings support the potential of miRNA panels as biomarkers for movement disorders and may provide more insights into the pathological mechanisms related to these disorders.
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DOI：
10.1136/jnnp-2013-307539
发表时间：
2014-10
期刊：
Journal of neurology, neurosurgery, and psychiatry
影响因子：
--
作者：
Magdalinou N;Lees AJ;Zetterberg H
通讯作者：
Zetterberg H
影响因子：
56.9
作者：
Kim, Jongpil;Inoue, Keiichi;Abeliovich, Asa
通讯作者：
Abeliovich, Asa
影响因子：
11
作者：
HUGHES, AJ;DANIEL, SE;LEES, AJ
通讯作者：
LEES, AJ
影响因子：
3.5
作者：
Minones-Moyano, Elena;Porta, Silvia;Marti, Eulalia
通讯作者：
Marti, Eulalia
影响因子：
168.9
作者：
Kalia, Lorraine V.;Lang, Anthony E.
通讯作者：
Lang, Anthony E.