Neural tube derived Wnt signals cooperate with FGF signaling in the formation and differentiation of the trigeminal placodes.
Neural tube derived Wnt signals cooperate with FGF signaling in the formation and differentiation of the trigeminal placodes.
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DOI：
10.1186/1749-8104-3-35
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发表时间：
2008-12-15
影响因子：
3.6
通讯作者：
Jones CM
中科院分区：
文献类型：
--
作者：
Canning CA;Lee L;Luo SX;Graham A;Jones CM
神经源性胎盘是脊椎动物头部形成的胚胎外胚层的局灶性增厚。正是在这些结构中，脑神经节的大多数感觉神经元的前体被指定。三叉神经节，眼球和上颌骨，形成在中脑-后脑边界附近，许多证据表明，从这一水平的神经轴发出的信号对眼球节节的发育非常重要。在这里，我们提供了第一个证据，表明眼部和上颌骨的胎盘都是在峡部WNT和成纤维细胞生长因子信号的影响下形成的。激活的Wnt信号引导表达Pax3的眼部胎盘区的发育，并诱导眼部和上颌部胎盘的过早分化。类似地，Fgf8的过度表达引导三叉神经安慰剂的过早分化。WNT信号需要成纤维细胞生长因子受体的活性来启动Pax3的表达，随后在颅骨外胚层中表达神经标记物，如Brn3a。此外，成纤维细胞生长因子信号通过丝裂原活化蛋白激酶通路是维持三叉神经安慰剂早期神经元分化所必需的。我们证明了三叉神经节形成所必需的感应信号的同一性。这是第一个描述峡部来源的Wnt信号如何与成纤维细胞生长因子信号协同作用的报告。两者加在一起，对于建立和分化眼部和上颌下颌部的基底节以及三叉神经节是必要和充分的。
Neurogenic placodes are focal thickenings of the embryonic ectoderm that form in the vertebrate head. It is within these structures that the precursors of the majority of the sensory neurons of the cranial ganglia are specified. The trigeminal placodes, the ophthalmic and maxillomandibular, form close to the midbrain-hindbrain boundary and many lines of evidence have shown that signals emanating from this level of the neuraxis are important for the development of the ophthalmic placode. Here, we provide the first evidence that both the ophthalmic and maxillomandibular placodes form under the influence of isthmic Wnt and FGF signals. Activated Wnt signals direct development of the Pax3 expressing ophthalmic placodal field and induce premature differentiation of both the ophthalmic and the maxillomandibular placodes. Similarly, overexpression of Fgf8 directs premature differentiation of the trigeminal placodes. Wnt signals require FGF receptor activity to initiate Pax3 expression and, subsequently, the expression of neural markers, such as Brn3a, within the cranial ectoderm. Furthermore, fibroblast growth factor signaling via the mitogen activated protein kinase pathway is required to maintain early neuronal differentiation within the trigeminal placodes. We demonstrate the identity of inductive signals that are necessary for trigeminal ganglion formation. This is the first report that describes how isthmic derived Wnt signals act in concert with fibroblast growth factor signaling. Together, both are necessary and sufficient for the establishment and differentiation of the ophthalmic and maxillomandibular placodes and, consequently, the trigeminal ganglion.
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影响因子：
10.5
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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Pourquié, O
DOI：
10.1016/j.bbrc.2003.09.158
发表时间：
2003-11-07
影响因子：
3.1
作者：
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