Analysis of differentially expressed genes and signaling pathways related to intramuscular fat deposition in skeletal muscle of sex-linked dwarf chickens.
Analysis of differentially expressed genes and signaling pathways related to intramuscular fat deposition in skeletal muscle of sex-linked dwarf chickens.
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伴性侏儒鸡骨骼肌肌内脂肪沉积相关差异表达基因及信号通路分析
DOI：
10.1155/2014/724274
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发表时间：
2014
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Ye Y;Lin S;Mu H;Tang X;Ou Y;Chen J;Ma Y;Li Y
肌内脂肪(IMF)在肉品品质中起着重要作用。然而，性连锁矮小(SLD)鸡骨骼肌中IMF沉积的分子机制还没有得到解决。本研究利用7周龄SLD鸡和正常鸡的基因表达谱，对鸡腿肌组织中与IMF沉积相关的潜在候选基因和信号通路进行了研究。共有173个差异表达基因(Deg)在两个品种之间被鉴定。随后，基于基因本体论(GO)分析，在每个品种中验证了与脂肪代谢或肌肉发育相关的6个基因。此外，DEGS的KEGG通路分析表明，它们中的一些(GHR、SOCS3和IGF2BP3)参与了脂肪细胞因子和胰岛素信号转导通路。为探讨上述信号通路在IMF沉积中的作用，采用定量逆转录聚合酶链式反应(qRT-PCR)和免疫印迹分析(Western印迹)方法检测了上述信号通路因子及下游基因的表达。总之，这些结果确定了与脂肪沉积相关的潜在候选基因，并表明脂肪细胞因子和胰岛素途径以及其他下游信号通路(转化生长因子-β/Smad3和Wnt/连环蛋白-β途径)在一定程度上调节了系统性红斑狼疮鸡骨骼肌中脂肪沉积。
Intramuscular fat (IMF) plays an important role in meat quality. However, the molecular mechanisms underlying IMF deposition in skeletal muscle have not been addressed for the sex-linked dwarf (SLD) chicken. In this study, potential candidate genes and signaling pathways related to IMF deposition in chicken leg muscle tissue were characterized using gene expression profiling of both 7-week-old SLD and normal chickens. A total of 173 differentially expressed genes (DEGs) were identified between the two breeds. Subsequently, 6 DEGs related to lipid metabolism or muscle development were verified in each breed based on gene ontology (GO) analysis. In addition, KEGG pathway analysis of DEGs indicated that some of them (GHR, SOCS3, and IGF2BP3) participate in adipocytokine and insulin signaling pathways. To investigate the role of the above signaling pathways in IMF deposition, the gene expression of pathway factors and other downstream genes were measured by using qRT-PCR and Western blot analyses. Collectively, the results identified potential candidate genes related to IMF deposition and suggested that IMF deposition in skeletal muscle of SLD chicken is regulated partially by pathways of adipocytokine and insulin and other downstream signaling pathways (TGF-β/SMAD3 and Wnt/catenin-β pathway).
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