Differential gene expression in ovaries of Qira black sheep and Hetian sheep using RNA-Seq technique.
Differential gene expression in ovaries of Qira black sheep and Hetian sheep using RNA-Seq technique.
复制标题
利用RNA-Seq技术研究齐拉黑羊和和田羊卵巢中的差异基因表达。
DOI：
10.1371/journal.pone.0120170
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zeng XC
中科院分区：
文献类型：
--
作者：
Chen HY;Shen H;Jia B;Zhang YS;Wang XH;Zeng XC
策勒黑羊和和田羊是我国西北地区的两个地方品种，分别具有高繁殖力和低繁殖力的特点。阐明不同绵羊品种卵巢中代表繁殖力极端的mRNA表达谱，将有助于绵羊主要繁殖力基因的鉴定和利用。利用RNA-seq技术比较策勒黑羊和和田羊卵巢mRNA表达谱的差异。从策勒黑羊和和田羊文库中，我们分别获得了总计11，747，582和11，879，968个测序读段。与参考序列比对后，两个库分别包含16，763个和16，814个基因。结果表明，和田羊与策勒黑羊共检测到1,252个基因的差异表达。随机选取8个差异表达基因进行实时荧光定量PCR验证。本研究为今后绵羊繁殖研究提供了基础资料。
The Qira black sheep and the Hetian sheep are two local breeds in the Northwest of China, which are characterized by high-fecundity and low-fecundity breed respectively. The elucidation of mRNA expression profiles in the ovaries among different sheep breeds representing fecundity extremes will helpful for identification and utilization of major prolificacy genes in sheep. In the present study, we performed RNA-seq technology to compare the difference in ovarian mRNA expression profiles between Qira black sheep and Hetian sheep. From the Qira black sheep and the Hetian sheep libraries, we obtained a total of 11,747,582 and 11,879,968 sequencing reads, respectively. After aligning to the reference sequences, the two libraries included 16,763 and 16,814 genes respectively. A total of 1,252 genes were significantly differentially expressed at Hetian sheep compared with Qira black sheep. Eight differentially expressed genes were randomly selected for validation by real-time RT-PCR. This study provides a basic data for future research of the sheep reproduction.
登录
查看更多内容
影响因子：
4.4
作者：
Driver AM;Peñagaricano F;Huang W;Ahmad KR;Hackbart KS;Wiltbank MC;Khatib H
通讯作者：
Khatib H
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
3.6
作者：
Greenaway, J;Gentry, PA;Petrik, JJ
通讯作者：
Petrik, JJ
影响因子：
2.2
作者：
Javanmard, Arash;Azadzadeh, N.;Esmailizadeh, Ali K.
通讯作者：
Esmailizadeh, Ali K.
影响因子：
3.4
作者：
Cannon, Matthew V;Takeda, Kumiko;Pinkert, Carl A
通讯作者：
Pinkert, Carl A