Genomic characterization of the Guillain-Barre syndrome-associated Campylobacter jejuni ICDCCJ07001 Isolate.
Genomic characterization of the Guillain-Barre syndrome-associated Campylobacter jejuni ICDCCJ07001 Isolate.
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DOI：
10.1371/journal.pone.0015060
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发表时间：
2010-11-29
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhang M;He L;Li Q;Sun H;Gu Y;You Y;Meng F;Zhang J
空肠弯曲菌ICDCCJ 07001（HS：41，ST 2993）是在36例格林-巴利综合征（GBS）暴发中从一例GBS患者中分离到的。2007年中国北方的空肠感染。序列分析表明，ICDCCJ 07001基因组由1，664，840个碱基对（bp）组成，四环素抗性质粒为44，084 bp。GC含量为59.29%，在染色体和质粒上分别鉴定出1,579个和37个CDS。将ICDCCJ 07001基因组与C.空肠亚种空肠81-176、81116、NCTC 11168、RM 1221和C.空肠亚种doylei 269.97。ICDCCJ 07001的长度和组织与NCTC 11168、81-176和81-116相似，除了CMLP 1在菌株ICDCCJ 07001中具有相反的取向。比较基因组分析也进行了GBS相关的C。空肠菌株。在4个GBS相关菌株中存在13个共同基因，9个基因定位到LOS簇，ICDCCJ07001_pTet（44 kb）质粒在结构上是嵌合的。ICDCCJ07001_pTet中的37个预测CDS与弯曲杆菌中的三种毒力相关质粒中存在的基因同源：81-176_pTet、pCC 31和81-176_pVir。毒力位点和毒力相关基因的比较分析表明，ICDCCJ 07001的LOS生物合成位点属于A型，以前报道的与GBS病例相关。ICDCCJ 07001的多糖荚膜生物合成（CPS）位点和鞭毛修饰（FM）位点与与菌株ICDCCJ 07001相似血清型的菌株260.94的相应序列相似。其它毒力相关基因cadF、peb 1、jlpA、cdt和ciaB在C.检查空肠菌株。
Campylobacter jejuni ICDCCJ07001 (HS:41, ST2993) was isolated from a Guillain-Barré syndrome (GBS) patient during a 36-case GBS outbreak triggered by C. jejuni infections in north China in 2007. Sequence analysis revealed that the ICDCCJ07001 genome consisted of 1,664,840 base pairs (bp) and one tetracycline resistance plasmid of 44,084 bp. The GC content was 59.29% and 1,579 and 37 CDSs were identified on the chromosome and plasmid, respectively. The ICDCCJ07001 genome was compared to C. jejuni subsp. jejuni strains 81-176, 81116, NCTC11168, RM1221 and C. jejuni subsp. doylei 269.97. The length and organization of ICDCCJ07001 was similar to that of NCTC11168, 81-176 and 81-116 except that CMLP1 had a reverse orientation in strain ICDCCJ07001. Comparative genomic analyses were also carried out between GBS-associated C. jejuni strains. Thirteen common genes were present in four GBS-associated strains and 9 genes mapped to the LOS cluster and the ICDCCJ07001_pTet (44 kb) plasmid was mosaic in structure. Thirty-seven predicted CDS in ICDCCJ07001_pTet were homologous to genes present in three virulence-associated plasmids in Campylobacter: 81-176_pTet, pCC31 and 81-176_pVir. Comparative analysis of virulence loci and virulence-associated genes indicated that the LOS biosynthesis loci of ICDCCJ07001 belonged to type A, previously reported to be associated with cases of GBS. The polysaccharide capsular biosynthesis (CPS) loci and the flagella modification (FM) loci of ICDCCJ07001 were similar to corresponding sequences of strain 260.94 of similar serotype as strain ICDCCJ07001. Other virulence-associated genes including cadF, peb1, jlpA, cdt and ciaB were conserved between the C. jejuni strains examined.
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