An A20/AN1-type zinc finger protein modulates gibberellins and abscisic acid contents and increases sensitivity to abiotic stress in rice (Oryza sativa).
An A20/AN1-type zinc finger protein modulates gibberellins and abscisic acid contents and increases sensitivity to abiotic stress in rice (Oryza sativa).
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A20/AN1 型锌指蛋白可调节赤霉素和脱落酸含量，并提高水稻 (Oryza sativa) 对非生物胁迫的敏感性。
DOI：
10.1093/jxb/erv464
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发表时间：
2016
影响因子：
6.9
通讯作者：
Huang Ji
中科院分区：
文献类型：
--
作者：
Zhang Ye;Lan Hongxia;Shao Qiaolin;Wang Ruqin;Chen Hui;Tang Haijuan;Zhang Hongsheng;Huang Ji
植物激素赤霉素（GA）和脱落酸（阿坝）在植物生长发育和逆境反应中起着重要作用。本文报道了一种新的A20/AN 1型锌指蛋白ZFP 185，它参与GA和阿坝信号转导，调控植物的生长和胁迫反应。ZFP 185在水稻不同组织中组成型表达。在水稻中过量表达ZFP 185导致半矮秆表型、细胞大小减小和内源GA 3含量降低。相比之下，在RNAi植物中观察到更高的GA 3含量。外源GA 3的应用可以完全挽救ZFP 185过表达植株的半矮化表型，表明ZFP 185在GA生物合成中起负作用。除GA外，ZFP 185的过表达降低了阿坝含量和几个阿坝生物合成相关基因的表达。此外，发现ZFP 185，不像以前已知的A20/AN 1型锌指基因，增加对干旱，寒冷和盐胁迫的敏感性，这意味着ZFP 185在胁迫耐受性中的负面作用。ZFP 185定位于细胞质中，并且缺乏转录激活潜力。我们的研究表明，ZFP 185通过影响GA和阿坝的生物合成来调节植物的生长和胁迫反应。
The plant hormones gibberellins (GA) and abscisic acid (ABA) play important roles in plant development and stress responses. Here we report a novel A20/AN1-type zinc finger protein ZFP185 involved in GA and ABA signaling in the regulation of growth and stress response. ZFP185 was constitutively expressed in various rice tissues. Overexpression of ZFP185 in rice results in a semi-dwarfism phenotype, reduced cell size, and the decrease of endogenous GA3 content. By contrast, higher GA3 content was observed in RNAi plants. The application of exogenous GA3 can fully rescue the semi-dwarfism phenotype of ZFP185 overexpressing plants, suggesting the negative role of ZFP185 in GA biosynthesis. Besides GA, overexpression of ZFP185 decreased ABA content and expression of several ABA biosynthesis-related genes. Moreover, it was found that ZFP185, unlike previously known A20/AN1-type zinc finger genes, increases sensitivity to drought, cold, and salt stresses, implying the negative role of ZFP185 in stress tolerance. ZFP185 was localized in the cytoplasm and lacked transcriptional activation potential. Our study suggests that ZFP185 regulates plant growth and stress responses by affecting GA and ABA biosynthesis in rice.
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DOI：
--
发表时间：
2004
期刊：
--
影响因子：
--
作者：
L. Ge;Hui Chen;Jiafu Jiang;Yuan Zhao;Ming-Li Xu;Yunyuan Xu;Ke-hui Tan;Zhi-hong Xu;K. Chong
通讯作者：
L. Ge;Hui Chen;Jiafu Jiang;Yuan Zhao;Ming-Li Xu;Yunyuan Xu;Ke-hui Tan;Zhi-hong Xu;K. Chong
影响因子：
4.5
作者：
Yaish MW;El-Kereamy A;Zhu T;Beatty PH;Good AG;Bi YM;Rothstein SJ
通讯作者：
Rothstein SJ
影响因子：
4.9
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
13.8
作者：
K. Heyninck;R. Beyaert
通讯作者：
K. Heyninck;R. Beyaert
影响因子：
5.1
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K