The complete mitochondrial genome of the damsel bug Alloeorhynchus bakeri (Hemiptera: Nabidae).

The complete mitochondrial genome of the damsel bug Alloeorhynchus bakeri (Hemiptera: Nabidae).
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DOI:
10.7150/ijbs.8.93
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发表时间:
2012
影响因子:
9.2
通讯作者:
Cai W
Cai W
中科院分区:
生物学2区
文献类型:
--
作者:
Li H;Liu H;Cao L;Shi A;Yang H;Cai W

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本研究已完成并注释了异卵小虫(Alloeorhynchus bakeri)线粒体DNA的完整序列。它代表了异翅龙科的第一个线粒体基因组测序。圆形基因组长15851 bp, A+T含量为73.5%,包含与六足动物祖先相同的37个典型基因。核苷酸组成和密码子的使用与其他已知的异翅目线粒体基因组相似。所有蛋白编码基因(pcg)都使用标准起始密码子(蛋氨酸和异亮氨酸),但COI以TTG起始。典型的TAA和TAG终止密码子存在于8个蛋白编码基因中,其余5个(COI、COII、COIII、ND5、ND1)具有不完全终止密码子(T或TA)。两条链的PCGs呈现相反的CG倾斜,这也反映在核苷酸组成和密码子的使用上。所有trna都具有典型的三叶草结构,除了tRNASer (AGN)的二氢吡啶(东华大学)臂形成了许多其他后生动物中已知的简单环。bakeri的核糖体RNA基因的二级结构模型,类似于那些提出了其他昆虫目。rrnL和rrnS的二级结构分别有6个结构域和45个螺旋,3个结构域和27个螺旋。小核糖体亚基与tRNAIle基因之间的主要非编码区(也称为控制区)包括两个特殊区域。第一个区域包括4个133 bp的串联重复单元和一个重复的部分拷贝(开头28 bp),第二个区域位于控制区末端,包含4个潜在的茎环结构。最后,利用PCGs序列进行系统发育研究。极大似然分析和贝叶斯推断分析均高度支持纳比达科是花尾科和密尾科的姐妹类群。
The complete sequence of the mitochondrial DNA (mtDNA) of the damsel bug, Alloeorhynchus bakeri, has been completed and annotated in this study. It represents the first sequenced mitochondrial genome of heteropteran family Nabidae. The circular genome is 15, 851 bp in length with an A+T content of 73.5%, contains the typical 37 genes that are arranged in the same order as that of the putative ancestor of hexapods. Nucleotide composition and codon usage are similar to other known heteropteran mitochondrial genomes. All protein-coding genes (PCGs) use standard initiation codons (methionine and isoleucine), except COI, which started with TTG. Canonical TAA and TAG termination codons are found in eight protein-coding genes, the remaining five (COI, COII, COIII, ND5, ND1) have incomplete termination codons (T or TA). PCGs of two strands present opposite CG skew which is also reflected by the nucleotide composition and codon usage. All tRNAs have the typical clover-leaf structure, except the dihydrouridine (DHU) arm of tRNASer (AGN)which forms a simple loop as known in many other metazoa. Secondary structure models of the ribosomal RNA genes of A. bakeri are presented, similar to those proposed for other insect orders. There are six domains and 45 helices and three domains and 27 helices in the secondary structures of rrnL and rrnS, respectively. The major non-coding region (also called control region) between the small ribosomal subunit and the tRNAIle gene includes two special regions. The first region includes four 133 bp tandem repeat units plus a partial copy of the repeat (28 bp of the beginning), and the second region at the end of control region contains 4 potential stem-loop structures. Finally, PCGs sequences were used to perform a phylogenetic study. Both maximum likelihood and Bayesian inference analyses highly support Nabidae as the sister group to Anthocoridae and Miridae.
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