Long-Range Interactions in Riboswitch Control of Gene Expression.
Long-Range Interactions in Riboswitch Control of Gene Expression.
复制标题
DOI：
10.1146/annurev-biophys-070816-034042
复制
发表时间：
2017-05-22
影响因子：
12.4
通讯作者：
Ferré-D'Amaré AR
中科院分区：
文献类型：
--
作者：
Jones CP;Ferré-D'Amaré AR
核糖开关是广泛存在的RNA基序，其响应于波动的代谢物浓度来调节基因表达。已知主要来自细菌，核糖开关将特异性配体结合和RNA结构的变化顺式偶联到mRNA表达。大多数在遗传学上广泛分布的核糖开关类的配体结合结构域的晶体结构，每一种都对特定的代谢物或离子具有特异性，现在已经可以获得。因此，结合态-一个终点-已经彻底的特点，但非结合态一直更难以捉摸。因此，未结合的传感核糖开关如何重新折叠成配体结合诱导的输出状态尚不清楚。核糖开关的配体识别机制是多种多样的，但我们发现，它们有一个共同的结构策略，将它们的结合位点定位在RNA三维折叠的点上，在该点上，序列中彼此相距最远的残基相遇。我们回顾核糖开关折叠如何坚持这一基本策略，并提出未来的研究方向，以了解和利用其特异性控制基因表达的能力。
Riboswitches are widespread RNA motifs that regulate gene expression in response to fluctuating metabolite concentrations. Known primarily from bacteria, riboswitches couple specific ligand binding and changes in RNA structure to mRNA expression in cis. Crystal structures of the ligand-binding domains of most of the phylogenetically widespread classes of riboswitches, each specific to a particular metabolite or ion, are now available. Thus, the bound states—one end point—have been thoroughly characterized, but the unbound states have been more elusive. Consequently, how the unbound, sensing riboswitch refolds into ligand-binding induced output state is less clear. The ligand recognition mechanisms of riboswitches are diverse, but we find that they share a common structural strategy in positioning their binding sites at the point of the RNA three-dimensional fold where the residues farthest from one another in sequence meet. We review how riboswitch folds adhere to this fundamental strategy and propose future research directions to understand and harness their ability to specifically control gene expression.
登录
查看更多内容
影响因子：
14.9
作者：
Espah Borujeni A;Mishler DM;Wang J;Huso W;Salis HM
通讯作者：
Salis HM
影响因子：
--
作者：
Ames TD;Rodionov DA;Weinberg Z;Breaker RR
通讯作者：
Breaker RR
影响因子：
16.6
作者：
Baird, Nathan J.;Inglese, James;Ferre-D'Amare, Adrian R.
通讯作者：
Ferre-D'Amare, Adrian R.
影响因子：
6.1
作者：
Batey, Robert T.
通讯作者：
Batey, Robert T.
影响因子：
7.3
作者：
Batey, Robert T.
通讯作者：
Batey, Robert T.