Proteome dynamics and physiological responses to short-term salt stress in Leymus chinensis leaves.
Proteome dynamics and physiological responses to short-term salt stress in Leymus chinensis leaves.
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羊草叶蛋白质组动力学和短期盐胁迫的生理反应
DOI：
10.1371/journal.pone.0183615
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Qu M
中科院分区：
文献类型：
--
作者：
Li J;Cui G;Hu G;Wang M;Zhang P;Qin L;Shang C;Zhang H;Zhu X;Qu M
盐胁迫正对全球农业构成越来越大的威胁。本研究以耐盐牧草羊草为材料，对其进行了生理和蛋白质组学分析。本研究的目的是了解羊草耐盐性的潜在机制，为作物分子育种提供依据。进行了一系列短期(<；48h)氯化钠处理(0~700 mm)。生理指标表明，盐胁迫抑制了根和叶的生长，降低了叶片的叶绿素含量，同时积累了导水率、脯氨酸、糖和蔗糖。对于蛋白质组学分析，我们获得了274个响应于盐处理的差异表达蛋白质。GO分析表明，274个蛋白质中有44个参与了氨基酸的生物合成和碳代谢。我们的研究结果表明，羊草通过刺激POD、SOD和CAT酶的活性，加速柠檬酸循环后期的反应，以及脯氨酸和糖的合成来应对盐胁迫。蛋白质组学分析也表明，盐胁迫抑制了光系统相关蛋白的表达、Calvin循环和叶绿体的生物合成，这与我们的生理数据一致。
Salt stress is becoming an increasing threat to global agriculture. In this study, physiological and proteomics analysis were performed using a salt-tolerant grass species, Leymus chinensis (L. chinensis). The aim of this study is to understand the potential mechanism of salt tolerance in L. chinensis that used for crop molecular breeding. A series of short-term (<48 h) NaCl treatments (0 ~ 700 mM) were conducted. Physiological data indicated that the root and leaves growth were inhibited, chlorophyll contents decreased, while hydraulic conductivity, proline, sugar and sucrose were accumulated under salt stress. For proteomic analysis, we obtained 274 differentially expressed proteins in response to NaCl treatments. GO analysis revealed that 44 out of 274 proteins are involved in the biosynthesis of amino acids and carbon metabolism. Our findings suggested that L. chinensis copes with salt stress by stimulating the activities of POD, SOD and CAT enzymes, speeding up the reactions of later steps of citrate cycle, and synthesis of proline and sugar. In agreement with our physiological data, proteomic analysis also showed that salt stress depress the expression of photosystem relevant proteins, Calvin cycle, and chloroplast biosynthesis.
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影响因子：
14.9
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影响因子：
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作者：
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