Molecular determination of antimicrobial resistance in Escherichia coli isolated from raw meat in Addis Ababa and Bishoftu, Ethiopia.
Molecular determination of antimicrobial resistance in Escherichia coli isolated from raw meat in Addis Ababa and Bishoftu, Ethiopia.
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DOI：
10.1186/s12941-017-0233-x
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发表时间：
2017-08-15
影响因子：
5.7
通讯作者：
Werid GM
中科院分区：
文献类型：
--
作者：
Messele YE;Abdi RD;Yalew ST;Tegegne DT;Emeru BA;Werid GM
食用被E.大肠杆菌会导致严重的疾病，甚至死亡。近年来，食源性大肠杆菌耐药株的出现使其耐药性日益严重。大肠杆菌对全球的公共卫生构成严重威胁。然而，关于大肠杆菌的抗生素耐药性特征知之甚少。埃塞俄比亚的大肠杆菌。本研究旨在了解大肠埃希菌的流行情况及耐药性。大肠杆菌分离自不同类型的肉类。从2015年12月至2016年4月，从屠宰场共采集了292份样本，以确定E.从埃塞俄比亚阿迪斯和Bishoftu的生牛肉、羊肉、羊肉和鸡肉中分离的大肠杆菌。针对埃塞俄比亚市场上流通的常用抗生素，对分离株进行AMR筛选。表型和基因型方法分别采用纸片扩散法和PCR法检测AMR。大肠埃希菌检出率为21.6%（63/5）。杆菌其中，E.鸡肉中检出大肠杆菌27株（37.0%），其次为羊肉17株（23.3%）、羊肉15株（20.6%）和牛肉4株（5.5%）。对63株分离菌的纸片扩散试验结果表明，对所有抗菌药物均不耐药的菌株仅占4.8%。多重耐药（耐≥3种药物）占46.0%。对氨苄西林和四环素的耐药率分别为71.4%和47.6%。还使用PCR鉴定了与AMR相关的基因。携带四环素类（泰特（A））、β-内酰胺类（blaCMY）和磺胺类（sulI）耐药基因的大肠埃希菌检出率分别为65.1%、65.1%和54.0%。63株大肠埃希菌中有25株（39.7%）携带有3种或3种以上药物的耐药基因。还确定了抗生素耐药表型和耐药基因的关联。对四环素、磺胺甲唑和氯霉素的抗性无论是表型还是基因型检测都很高。阻力E的上升水平。大肠杆菌对多种抗菌剂的耐受性决定了迫切需要规范和监测动物和人类的抗菌剂使用。
Consumption of meat contaminated by E. coli causes a serious illness and even death to affected individuals. Recently the emerging of antibiotic resistant foodborne E. coli poses serious public health risks worldwide. However, little is known about the antibiotic resistance profile of E. coli in Ethiopia. This study aimed to determine the prevalence and Antimicrobial resistance (AMR) status of E. coli isolated from different type of meat. Overall 292 samples were collected from December 2015 to April 2016 from slaughterhouses to determine the prevalence and AMR of E. coli isolated from raw beef, mutton, chevon and chicken meat from Addis Ababa and Bishoftu, Ethiopia. The isolates were screened for AMR against commonly used antibiotics circulating in the Ethiopian market. Both phenotypic and genotypic approach were employed for AMR detection using disc diffusion test and PCR respectively. The prevalence of E. coli was 63 (21.6%), indicating one sample in every five samples harbors E. coli. Among these, the highest E. coli isolates was observed in chicken meat samples (37.0%; 27), followed by mutton (23.3%; 17), chevon (20.6%; 15) and beef (5.5%; 4). Results of disk diffusion test on the 63 isolates showed that only 4.8% of them were not resistance to all antimicrobials tested. Multiple drug resistance (resistance to ≥3 drugs) was 46.0%. Significantly high resistance to ampicillin (71.4%) and tetracycline (47.6%) was observed. Identification of genes associated with AMR was also done using PCR. The prevalence of E. coli isolates harboring resistance gene responsible for tetracycline (tet(A)), beta lactams (blaCMY) and sulphanamide (sulI) antibiotics were found 65.1, 65.1 and 54.0%, respectively. Twenty-five out of the 63 (39.7% %) E. coli isolates have got antimicrobial resistance gene to three or more classes of drugs. The associations of antimicrobial resistance phenotypes and resistance genes was also determined. The detection of resistance trait against tetracycline, sulphametazole and chloramphenicol measured either phenotypically or genotypically were high. The rising levels of resistance E. coli to multiple antimicrobial dictate the urgent need to regulate and monitor antimicrobial use in both animals and humans.
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影响因子：
5.2
作者：
Frye JG;Jackson CR
通讯作者：
Jackson CR
DOI：
10.1111/j.1439-0450.2004.00788.x
发表时间：
2004-10-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Molbak, K
通讯作者：
Molbak, K
影响因子：
9.4
作者：
Pradel, N;Livrelli, V;Forestier, C
通讯作者：
Forestier, C
影响因子：
2.4
作者：
Fode-Vaughan, KA;Maki, JS;Collins, MLP
通讯作者：
Collins, MLP
影响因子：
--
作者：
Bitew, Molalegne;Tafere, Arega;Tolosa, Tadele
通讯作者：
Tolosa, Tadele